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|a Printing and erasing of DNA-based photoresists inside synthetic cells [research data]
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|a In the pursuit to produce functioning synthetic cells from the bottom up, DNA nanotechnology has proven to be a powerful tool. However, the crowded yet highly organized arrangement in living cells, bridging from the nano- to the micron-scale, remains challenging to recreate with DNA-based architectures. Here, laser microprinting is established to print and erase shape-controlled DNA hydrogels inside the confinement of water-in-oil droplets and giant unilamellar lipid vesicles (GUVs). The DNA-based photoresist consists of a photocleavable inactive DNA linker which interconnects Y-shaped DNA motifs when activated by local irradiation with a 405 nm laser. An alternative linker design allows to erase custom features from a preformed DNA hydrogel with feature sizes down to 1.38um. The present work demonstrates that the DNA hydrogels can serve as an internal support to stabilize non-spherical GUV shapes. Overall, DNA-based photoresists for laser printing in confinement allow to build up architectures on the interior of synthetic cells with light, which diversifies the toolbox of bottom-up synthetic biology.
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In the pursuit to produce functioning synthetic cells from the bottom up, DNA nanotechnology has proven to be a powerful tool. However, the crowded yet highly organized arrangement in living cells, bridging from the nano- to the micron-scale, remains challenging to recreate with DNA-based architectures. Here, laser microprinting is established to print and erase shape-controlled DNA hydrogels inside the confinement of water-in-oil droplets and giant unilamellar lipid vesicles (GUVs). The DNA-based photoresist consists of a photocleavable inactive DNA linker which interconnects Y-shaped DNA motifs when activated by local irradiation with a 405 nm laser. An alternative linker design allows to erase custom features from a preformed DNA hydrogel with feature sizes down to 1.38um. The present work demonstrates that the DNA hydrogels can serve as an internal support to stabilize non-spherical GUV shapes. Overall, DNA-based photoresists for laser printing in confinement allow to build up architectures on the interior of synthetic cells with light, which diversifies the toolbox of bottom-up synthetic biology. |
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Göpfrich, Kerstin VerfasserIn (DE-588)1185596852 (DE-627)1664987150 aut, Printing and erasing of DNA-based photoresists inside synthetic cells [research data] Kerstin Goepfrich, Tobias Walther, Heidelberg Universität 2022-02-28, 1 Online-Ressource (17 Files), Text txt rdacontent, Computerdaten cod rdacontent, Computermedien c rdamedia, Online-Ressource cr rdacarrier, Gesehen am 28.02.2022, In the pursuit to produce functioning synthetic cells from the bottom up, DNA nanotechnology has proven to be a powerful tool. However, the crowded yet highly organized arrangement in living cells, bridging from the nano- to the micron-scale, remains challenging to recreate with DNA-based architectures. Here, laser microprinting is established to print and erase shape-controlled DNA hydrogels inside the confinement of water-in-oil droplets and giant unilamellar lipid vesicles (GUVs). The DNA-based photoresist consists of a photocleavable inactive DNA linker which interconnects Y-shaped DNA motifs when activated by local irradiation with a 405 nm laser. An alternative linker design allows to erase custom features from a preformed DNA hydrogel with feature sizes down to 1.38um. The present work demonstrates that the DNA hydrogels can serve as an internal support to stabilize non-spherical GUV shapes. Overall, DNA-based photoresists for laser printing in confinement allow to build up architectures on the interior of synthetic cells with light, which diversifies the toolbox of bottom-up synthetic biology., Chemistry, Engineering, Health and Life Sciences, Medicine, Physics, Forschungsdaten (DE-588)1098579690 (DE-627)857755366 (DE-576)469182156 gnd-content, Datenbank (DE-588)4011119-2 (DE-627)106354256 (DE-576)208891943 gnd-content, Walther, Tobias 1973- VerfasserIn (DE-588)124113257 (DE-627)085645907 (DE-576)294025308 aut, https://doi.org/10.11588/data/MKOC9S Verlag Resolving-System kostenfrei Volltext, https://heidata.uni-heidelberg.de/dataset.xhtml?persistentId=doi:10.11588/data/MKOC9S Verlag kostenfrei Volltext, https://doi.org/10.11588/data/MKOC9S LFER, https://heidata.uni-heidelberg.de/dataset.xhtml?persistentId=doi:10.11588/data/MKOC9S LFER, LFER 2022-03-20T12:51:36Z |
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Göpfrich, Kerstin, Walther, Tobias, Printing and erasing of DNA-based photoresists inside synthetic cells [research data], In the pursuit to produce functioning synthetic cells from the bottom up, DNA nanotechnology has proven to be a powerful tool. However, the crowded yet highly organized arrangement in living cells, bridging from the nano- to the micron-scale, remains challenging to recreate with DNA-based architectures. Here, laser microprinting is established to print and erase shape-controlled DNA hydrogels inside the confinement of water-in-oil droplets and giant unilamellar lipid vesicles (GUVs). The DNA-based photoresist consists of a photocleavable inactive DNA linker which interconnects Y-shaped DNA motifs when activated by local irradiation with a 405 nm laser. An alternative linker design allows to erase custom features from a preformed DNA hydrogel with feature sizes down to 1.38um. The present work demonstrates that the DNA hydrogels can serve as an internal support to stabilize non-spherical GUV shapes. Overall, DNA-based photoresists for laser printing in confinement allow to build up architectures on the interior of synthetic cells with light, which diversifies the toolbox of bottom-up synthetic biology., Chemistry, Engineering, Health and Life Sciences, Medicine, Physics, Forschungsdaten, Datenbank |
| title |
Printing and erasing of DNA-based photoresists inside synthetic cells [research data] |
| title_auth |
Printing and erasing of DNA-based photoresists inside synthetic cells [research data] |
| title_full |
Printing and erasing of DNA-based photoresists inside synthetic cells [research data] Kerstin Goepfrich, Tobias Walther |
| title_fullStr |
Printing and erasing of DNA-based photoresists inside synthetic cells [research data] Kerstin Goepfrich, Tobias Walther |
| title_full_unstemmed |
Printing and erasing of DNA-based photoresists inside synthetic cells [research data] Kerstin Goepfrich, Tobias Walther |
| title_short |
Printing and erasing of DNA-based photoresists inside synthetic cells [research data] |
| title_sort |
printing and erasing of dna based photoresists inside synthetic cells research data |
| topic |
Chemistry, Engineering, Health and Life Sciences, Medicine, Physics, Forschungsdaten, Datenbank |
| topic_facet |
Chemistry, Engineering, Health and Life Sciences, Medicine, Physics, Forschungsdaten, Datenbank |
| url |
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