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Transplantation of neural precursor cells attenuates chronic immune environment in cervical spinal cord injury
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| Veröffentlicht in: | Frontiers in neurology 9(2018), Artikel-ID 428, Seite 1-12 |
|---|---|
| Personen und Körperschaften: | , , , , , , |
| Titel: | Transplantation of neural precursor cells attenuates chronic immune environment in cervical spinal cord injury/ Lennart Riemann, Alexander Younsi, Moritz Scherer, Guoli Zheng, Thomas Skutella, Andreas W. Unterberg and Klaus Zweckberger |
| Format: | E-Book-Kapitel |
| Sprache: | Englisch |
| veröffentlicht: |
08 June 2018
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| Gesamtaufnahme: |
: Frontiers in neurology, 9(2018), Artikel-ID 428, Seite 1-12
, volume:9 |
| Schlagwörter: | |
| Quelle: | Verbunddaten SWB Lizenzfreie Online-Ressourcen |
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| 245 | 1 | 0 | |a Transplantation of neural precursor cells attenuates chronic immune environment in cervical spinal cord injury |c Lennart Riemann, Alexander Younsi, Moritz Scherer, Guoli Zheng, Thomas Skutella, Andreas W. Unterberg and Klaus Zweckberger |
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| 520 | |a Inflammation after traumatic spinal cord injury (SCI) is non-resolving and thus still present in chronic injury stages. It plays a key role in the pathophysiology of SCI and has been associated with further neurodegeneration and development of neuropathic pain. Neural precursor cells (NPCs) have been shown to reduce the acute and sub-acute inflammatory response after SCI. In the present study, we examined effects of NPC transplantation on the immune environment in chronic stages of SCI. SCI was induced in rats by clip-compression of the cervical spinal cord at the level C6-C7. NPCs were transplanted 10 days post-injury. The functional outcome was assessed weekly for eight weeks using the Basso, Beattie, and Bresnahan scale, the CatWalk system, and the grid walk test. Afterwards, the rats were sacrificed, and spinal cord sections were examined for M1/M2 macrophages, T lymphocytes, astrogliosis, and apoptosis using immunofluorescence staining. Rats treated with NPCs had compared to the control group significantly fewer pro-inflammatory M1 macrophages and reduced immunodensity for inducible nitric oxide synthase (iNOS), their marker enzyme. Anti-inflammatory M2 macrophages were rarely present eight weeks after the SCI. In this model, the sub-acute transplantation of NPCs did not support survival and proliferation of M2 macrophages. Post-traumatic apoptosis, however, was significantly reduced in the NPC group, which might be explained by the altered microenvironment following NPC transplantation. Corresponding to these findings, reactive astrogliosis was significantly reduced in NPC-transplanted animals. Furthermore, we could observe a trend towards smaller cavity sizes and functional improvement following NPC transplantation. Our data suggest that transplantation of NPCs following SCI might attenuate inflammation even in chronic injury stages. This might prevent further neurodegeneration and could also set a stage for improved neuroregeneration after SCI. | ||
| 650 | 4 | |a Apoptosis | |
| 650 | 4 | |a chronic inflammation | |
| 650 | 4 | |a Macrophages | |
| 650 | 4 | |a Neural precursor cell (NPC) | |
| 650 | 4 | |a Neuroregeneration | |
| 650 | 4 | |a spinal cord injury (SCI) | |
| 650 | 4 | |a Stem Cells | |
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| author | Riemann, Lennart, Younsi, Alexander, Scherer, Moritz, Zheng, Guoli, Skutella, Thomas, Unterberg, Andreas, Zweckberger, Klaus |
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| contents | Inflammation after traumatic spinal cord injury (SCI) is non-resolving and thus still present in chronic injury stages. It plays a key role in the pathophysiology of SCI and has been associated with further neurodegeneration and development of neuropathic pain. Neural precursor cells (NPCs) have been shown to reduce the acute and sub-acute inflammatory response after SCI. In the present study, we examined effects of NPC transplantation on the immune environment in chronic stages of SCI. SCI was induced in rats by clip-compression of the cervical spinal cord at the level C6-C7. NPCs were transplanted 10 days post-injury. The functional outcome was assessed weekly for eight weeks using the Basso, Beattie, and Bresnahan scale, the CatWalk system, and the grid walk test. Afterwards, the rats were sacrificed, and spinal cord sections were examined for M1/M2 macrophages, T lymphocytes, astrogliosis, and apoptosis using immunofluorescence staining. Rats treated with NPCs had compared to the control group significantly fewer pro-inflammatory M1 macrophages and reduced immunodensity for inducible nitric oxide synthase (iNOS), their marker enzyme. Anti-inflammatory M2 macrophages were rarely present eight weeks after the SCI. In this model, the sub-acute transplantation of NPCs did not support survival and proliferation of M2 macrophages. Post-traumatic apoptosis, however, was significantly reduced in the NPC group, which might be explained by the altered microenvironment following NPC transplantation. Corresponding to these findings, reactive astrogliosis was significantly reduced in NPC-transplanted animals. Furthermore, we could observe a trend towards smaller cavity sizes and functional improvement following NPC transplantation. Our data suggest that transplantation of NPCs following SCI might attenuate inflammation even in chronic injury stages. This might prevent further neurodegeneration and could also set a stage for improved neuroregeneration after SCI. |
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| spelling | Riemann, Lennart VerfasserIn (DE-588)1199567108 (DE-627)1681875551 aut, Transplantation of neural precursor cells attenuates chronic immune environment in cervical spinal cord injury Lennart Riemann, Alexander Younsi, Moritz Scherer, Guoli Zheng, Thomas Skutella, Andreas W. Unterberg and Klaus Zweckberger, 08 June 2018, 12, Text txt rdacontent, Computermedien c rdamedia, Online-Ressource cr rdacarrier, Gesehen am 14.11.2019, Inflammation after traumatic spinal cord injury (SCI) is non-resolving and thus still present in chronic injury stages. It plays a key role in the pathophysiology of SCI and has been associated with further neurodegeneration and development of neuropathic pain. Neural precursor cells (NPCs) have been shown to reduce the acute and sub-acute inflammatory response after SCI. In the present study, we examined effects of NPC transplantation on the immune environment in chronic stages of SCI. SCI was induced in rats by clip-compression of the cervical spinal cord at the level C6-C7. NPCs were transplanted 10 days post-injury. The functional outcome was assessed weekly for eight weeks using the Basso, Beattie, and Bresnahan scale, the CatWalk system, and the grid walk test. Afterwards, the rats were sacrificed, and spinal cord sections were examined for M1/M2 macrophages, T lymphocytes, astrogliosis, and apoptosis using immunofluorescence staining. Rats treated with NPCs had compared to the control group significantly fewer pro-inflammatory M1 macrophages and reduced immunodensity for inducible nitric oxide synthase (iNOS), their marker enzyme. Anti-inflammatory M2 macrophages were rarely present eight weeks after the SCI. In this model, the sub-acute transplantation of NPCs did not support survival and proliferation of M2 macrophages. Post-traumatic apoptosis, however, was significantly reduced in the NPC group, which might be explained by the altered microenvironment following NPC transplantation. Corresponding to these findings, reactive astrogliosis was significantly reduced in NPC-transplanted animals. Furthermore, we could observe a trend towards smaller cavity sizes and functional improvement following NPC transplantation. Our data suggest that transplantation of NPCs following SCI might attenuate inflammation even in chronic injury stages. This might prevent further neurodegeneration and could also set a stage for improved neuroregeneration after SCI., Apoptosis, chronic inflammation, Macrophages, Neural precursor cell (NPC), Neuroregeneration, spinal cord injury (SCI), Stem Cells, Younsi, Alexander 1985- VerfasserIn (DE-588)104607508X (DE-627)775681547 (DE-576)39935882X aut, Scherer, Moritz 1984- VerfasserIn (DE-588)1026157684 (DE-627)726207845 (DE-576)371433983 aut, Zheng, Guoli 1989- VerfasserIn (DE-588)1199567183 (DE-627)1681875667 aut, Skutella, Thomas 1963- VerfasserIn (DE-588)120568225 (DE-627)080752047 (DE-576)180448390 aut, Unterberg, Andreas VerfasserIn (DE-588)1032681187 (DE-627)738641200 (DE-576)168441233 aut, Zweckberger, Klaus 1978- VerfasserIn (DE-588)13205034X (DE-627)517299534 (DE-576)298915510 aut, Enthalten in Frontiers in neurology Lausanne : Frontiers Research Foundation, 2008 9(2018), Artikel-ID 428, Seite 1-12 Online-Ressource (DE-627)631498753 (DE-600)2564214-5 (DE-576)326550933 1664-2295 nnns, volume:9 year:2018 elocationid:428 pages:1-12 extent:12, https://doi.org/10.3389/fneur.2018.00428 Verlag Resolving-System kostenfrei Volltext, https://www.frontiersin.org/articles/10.3389/fneur.2018.00428/full Verlag kostenfrei Volltext, https://doi.org/10.3389/fneur.2018.00428 LFER, https://www.frontiersin.org/articles/10.3389/fneur.2018.00428/full LFER, LFER 2019-12-05T00:00:00Z |
| spellingShingle | Riemann, Lennart, Younsi, Alexander, Scherer, Moritz, Zheng, Guoli, Skutella, Thomas, Unterberg, Andreas, Zweckberger, Klaus, Transplantation of neural precursor cells attenuates chronic immune environment in cervical spinal cord injury, Inflammation after traumatic spinal cord injury (SCI) is non-resolving and thus still present in chronic injury stages. It plays a key role in the pathophysiology of SCI and has been associated with further neurodegeneration and development of neuropathic pain. Neural precursor cells (NPCs) have been shown to reduce the acute and sub-acute inflammatory response after SCI. In the present study, we examined effects of NPC transplantation on the immune environment in chronic stages of SCI. SCI was induced in rats by clip-compression of the cervical spinal cord at the level C6-C7. NPCs were transplanted 10 days post-injury. The functional outcome was assessed weekly for eight weeks using the Basso, Beattie, and Bresnahan scale, the CatWalk system, and the grid walk test. Afterwards, the rats were sacrificed, and spinal cord sections were examined for M1/M2 macrophages, T lymphocytes, astrogliosis, and apoptosis using immunofluorescence staining. Rats treated with NPCs had compared to the control group significantly fewer pro-inflammatory M1 macrophages and reduced immunodensity for inducible nitric oxide synthase (iNOS), their marker enzyme. Anti-inflammatory M2 macrophages were rarely present eight weeks after the SCI. In this model, the sub-acute transplantation of NPCs did not support survival and proliferation of M2 macrophages. Post-traumatic apoptosis, however, was significantly reduced in the NPC group, which might be explained by the altered microenvironment following NPC transplantation. Corresponding to these findings, reactive astrogliosis was significantly reduced in NPC-transplanted animals. Furthermore, we could observe a trend towards smaller cavity sizes and functional improvement following NPC transplantation. Our data suggest that transplantation of NPCs following SCI might attenuate inflammation even in chronic injury stages. This might prevent further neurodegeneration and could also set a stage for improved neuroregeneration after SCI., Apoptosis, chronic inflammation, Macrophages, Neural precursor cell (NPC), Neuroregeneration, spinal cord injury (SCI), Stem Cells |
| title | Transplantation of neural precursor cells attenuates chronic immune environment in cervical spinal cord injury |
| title_auth | Transplantation of neural precursor cells attenuates chronic immune environment in cervical spinal cord injury |
| title_full | Transplantation of neural precursor cells attenuates chronic immune environment in cervical spinal cord injury Lennart Riemann, Alexander Younsi, Moritz Scherer, Guoli Zheng, Thomas Skutella, Andreas W. Unterberg and Klaus Zweckberger |
| title_fullStr | Transplantation of neural precursor cells attenuates chronic immune environment in cervical spinal cord injury Lennart Riemann, Alexander Younsi, Moritz Scherer, Guoli Zheng, Thomas Skutella, Andreas W. Unterberg and Klaus Zweckberger |
| title_full_unstemmed | Transplantation of neural precursor cells attenuates chronic immune environment in cervical spinal cord injury Lennart Riemann, Alexander Younsi, Moritz Scherer, Guoli Zheng, Thomas Skutella, Andreas W. Unterberg and Klaus Zweckberger |
| title_in_hierarchy | Transplantation of neural precursor cells attenuates chronic immune environment in cervical spinal cord injury / Lennart Riemann, Alexander Younsi, Moritz Scherer, Guoli Zheng, Thomas Skutella, Andreas W. Unterberg and Klaus Zweckberger, |
| title_short | Transplantation of neural precursor cells attenuates chronic immune environment in cervical spinal cord injury |
| title_sort | transplantation of neural precursor cells attenuates chronic immune environment in cervical spinal cord injury |
| topic | Apoptosis, chronic inflammation, Macrophages, Neural precursor cell (NPC), Neuroregeneration, spinal cord injury (SCI), Stem Cells |
| topic_facet | Apoptosis, chronic inflammation, Macrophages, Neural precursor cell (NPC), Neuroregeneration, spinal cord injury (SCI), Stem Cells |
| url | https://doi.org/10.3389/fneur.2018.00428, https://www.frontiersin.org/articles/10.3389/fneur.2018.00428/full |