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An RNAi screen reveals an essential role for HIPK4 in human skin epithelial differentiation from iPSCs
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| Veröffentlicht in: | Stem cell reports 9(2017), 4, Seite 1234-1245 |
|---|---|
| Personen und Körperschaften: | , , , , , , |
| Titel: | An RNAi screen reveals an essential role for HIPK4 in human skin epithelial differentiation from iPSCs/ Lionel Larribère, Marta Galach, Daniel Novak, Karla Arévalo, Hans Christian Volz, Hans-Jürgen Stark, Petra Boukamp, Michael Boutros, and Jochen Utikal |
| Format: | E-Book-Kapitel |
| Sprache: | Englisch |
| veröffentlicht: |
October 10, 2017
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| Gesamtaufnahme: |
: Stem cell reports, 9(2017), 4, Seite 1234-1245
, volume:9 |
| Schlagwörter: | |
| Quelle: | Verbunddaten SWB Lizenzfreie Online-Ressourcen |
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| 245 | 1 | 3 | |a An RNAi screen reveals an essential role for HIPK4 in human skin epithelial differentiation from iPSCs |c Lionel Larribère, Marta Galach, Daniel Novak, Karla Arévalo, Hans Christian Volz, Hans-Jürgen Stark, Petra Boukamp, Michael Boutros, and Jochen Utikal |
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| 520 | |a Molecular mechanisms responsible for the development of human skin epithelial cells are incompletely understood. As a consequence, the efficiency to establish a pure skin epithelial cell population from human induced pluripotent stem cells (hiPSCs) remains poor. Using an approach including RNAi and high-throughput imaging of early epithelial cells, we identified candidate kinases involved in their differentiation from hiPSCs. Among these, we found HIPK4 to be an important inhibitor of this process. Indeed, its silencing increased the amount of generated skin epithelial precursors at an early time point, increased the amount of generated keratinocytes at a later time point, and improved growth and differentiation of organotypic cultures, allowing for the formation of a denser basal layer and stratification with the expression of several keratins. Our data bring substantial input regarding regulation of human skin epithelial differentiation and for improving differentiation protocols from pluripotent stem cells. | ||
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| 900 | |a Volz, Christian | ||
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| contents | Molecular mechanisms responsible for the development of human skin epithelial cells are incompletely understood. As a consequence, the efficiency to establish a pure skin epithelial cell population from human induced pluripotent stem cells (hiPSCs) remains poor. Using an approach including RNAi and high-throughput imaging of early epithelial cells, we identified candidate kinases involved in their differentiation from hiPSCs. Among these, we found HIPK4 to be an important inhibitor of this process. Indeed, its silencing increased the amount of generated skin epithelial precursors at an early time point, increased the amount of generated keratinocytes at a later time point, and improved growth and differentiation of organotypic cultures, allowing for the formation of a denser basal layer and stratification with the expression of several keratins. Our data bring substantial input regarding regulation of human skin epithelial differentiation and for improving differentiation protocols from pluripotent stem cells. |
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| spelling | Larribere, Lionel VerfasserIn (DE-588)1118655079 (DE-627)872164837 (DE-576)479578648 aut, An RNAi screen reveals an essential role for HIPK4 in human skin epithelial differentiation from iPSCs Lionel Larribère, Marta Galach, Daniel Novak, Karla Arévalo, Hans Christian Volz, Hans-Jürgen Stark, Petra Boukamp, Michael Boutros, and Jochen Utikal, October 10, 2017, 12, Text txt rdacontent, Computermedien c rdamedia, Online-Ressource cr rdacarrier, Gesehen am 30.08.2018, Molecular mechanisms responsible for the development of human skin epithelial cells are incompletely understood. As a consequence, the efficiency to establish a pure skin epithelial cell population from human induced pluripotent stem cells (hiPSCs) remains poor. Using an approach including RNAi and high-throughput imaging of early epithelial cells, we identified candidate kinases involved in their differentiation from hiPSCs. Among these, we found HIPK4 to be an important inhibitor of this process. Indeed, its silencing increased the amount of generated skin epithelial precursors at an early time point, increased the amount of generated keratinocytes at a later time point, and improved growth and differentiation of organotypic cultures, allowing for the formation of a denser basal layer and stratification with the expression of several keratins. Our data bring substantial input regarding regulation of human skin epithelial differentiation and for improving differentiation protocols from pluripotent stem cells., development, differentiation, epithelium, HIPK4, iPSC, keratinocytes, organotypic culture, RNAi screen, skin, stem cell, Novak, Daniel 1978- VerfasserIn (DE-588)1026462975 (DE-627)726763764 (DE-576)371815533 aut, Volz, Hans Christian 1977- VerfasserIn (DE-588)132862883 (DE-627)527874140 (DE-576)264553071 aut, Stark, Hans-Jürgen VerfasserIn (DE-588)1217623221 (DE-627)1731806809 aut, Boukamp, Petra VerfasserIn (DE-588)1205902406 (DE-627)1691750824 aut, Boutros, Michael VerfasserIn (DE-588)1023031132 (DE-627)717350045 (DE-576)366291831 aut, Utikal, Jochen 1974- VerfasserIn (DE-588)1026463750 (DE-627)726765015 (DE-576)371816580 aut, Enthalten in Stem cell reports Maryland Heights, MO : Cell Press, 2013 9(2017), 4, Seite 1234-1245 Online-Ressource (DE-627)749730862 (DE-600)2720528-9 (DE-576)383773474 2213-6711 nnns, volume:9 year:2017 number:4 pages:1234-1245 extent:12, http://dx.doi.org/10.1016/j.stemcr.2017.08.023 Verlag Resolving-System kostenfrei Volltext, http://www.sciencedirect.com/science/article/pii/S2213671117303806 Verlag kostenfrei Volltext, http://dx.doi.org/10.1016/j.stemcr.2017.08.023 LFER, LFER 2018-09-13T00:00:00Z |
| spellingShingle | Larribere, Lionel, Novak, Daniel, Volz, Hans Christian, Stark, Hans-Jürgen, Boukamp, Petra, Boutros, Michael, Utikal, Jochen, An RNAi screen reveals an essential role for HIPK4 in human skin epithelial differentiation from iPSCs, Molecular mechanisms responsible for the development of human skin epithelial cells are incompletely understood. As a consequence, the efficiency to establish a pure skin epithelial cell population from human induced pluripotent stem cells (hiPSCs) remains poor. Using an approach including RNAi and high-throughput imaging of early epithelial cells, we identified candidate kinases involved in their differentiation from hiPSCs. Among these, we found HIPK4 to be an important inhibitor of this process. Indeed, its silencing increased the amount of generated skin epithelial precursors at an early time point, increased the amount of generated keratinocytes at a later time point, and improved growth and differentiation of organotypic cultures, allowing for the formation of a denser basal layer and stratification with the expression of several keratins. Our data bring substantial input regarding regulation of human skin epithelial differentiation and for improving differentiation protocols from pluripotent stem cells., development, differentiation, epithelium, HIPK4, iPSC, keratinocytes, organotypic culture, RNAi screen, skin, stem cell |
| swb_id_str | 510557473 |
| title | An RNAi screen reveals an essential role for HIPK4 in human skin epithelial differentiation from iPSCs |
| title_auth | An RNAi screen reveals an essential role for HIPK4 in human skin epithelial differentiation from iPSCs |
| title_full | An RNAi screen reveals an essential role for HIPK4 in human skin epithelial differentiation from iPSCs Lionel Larribère, Marta Galach, Daniel Novak, Karla Arévalo, Hans Christian Volz, Hans-Jürgen Stark, Petra Boukamp, Michael Boutros, and Jochen Utikal |
| title_fullStr | An RNAi screen reveals an essential role for HIPK4 in human skin epithelial differentiation from iPSCs Lionel Larribère, Marta Galach, Daniel Novak, Karla Arévalo, Hans Christian Volz, Hans-Jürgen Stark, Petra Boukamp, Michael Boutros, and Jochen Utikal |
| title_full_unstemmed | An RNAi screen reveals an essential role for HIPK4 in human skin epithelial differentiation from iPSCs Lionel Larribère, Marta Galach, Daniel Novak, Karla Arévalo, Hans Christian Volz, Hans-Jürgen Stark, Petra Boukamp, Michael Boutros, and Jochen Utikal |
| title_in_hierarchy | An RNAi screen reveals an essential role for HIPK4 in human skin epithelial differentiation from iPSCs / Lionel Larribère, Marta Galach, Daniel Novak, Karla Arévalo, Hans Christian Volz, Hans-Jürgen Stark, Petra Boukamp, Michael Boutros, and Jochen Utikal, |
| title_short | An RNAi screen reveals an essential role for HIPK4 in human skin epithelial differentiation from iPSCs |
| title_sort | rnai screen reveals an essential role for hipk4 in human skin epithelial differentiation from ipscs |
| topic | development, differentiation, epithelium, HIPK4, iPSC, keratinocytes, organotypic culture, RNAi screen, skin, stem cell |
| topic_facet | development, differentiation, epithelium, HIPK4, iPSC, keratinocytes, organotypic culture, RNAi screen, skin, stem cell |
| url | http://dx.doi.org/10.1016/j.stemcr.2017.08.023, http://www.sciencedirect.com/science/article/pii/S2213671117303806 |