Eintrag weiter verarbeiten
Hydra mesoglea proteome identifies thrombospondin as a conserved component active in head organizer restriction
Gespeichert in:
| Veröffentlicht in: | Scientific reports 8(2018) Artikel-Nummer 11753, 18 Seiten |
|---|---|
| Personen und Körperschaften: | , , , |
| Titel: | Hydra mesoglea proteome identifies thrombospondin as a conserved component active in head organizer restriction/ Mark Lommel, Jennifer Strompen, Andrew L. Hellewell, Gnana Prakash Balasubramanian, Elena D. Christofidou, Andrew R. Thomson, Aimee L. Boyle, Derek N. Woolfson, Kane Puglisi, Markus Hartl, Thomas W. Holstein, Josephine C. Adams & Suat Özbek |
| Format: | E-Book-Kapitel |
| Sprache: | Englisch |
| veröffentlicht: |
06 August 2018
|
| Gesamtaufnahme: |
: Scientific reports, 8(2018) Artikel-Nummer 11753, 18 Seiten
, volume:8 |
| Quelle: | Verbunddaten SWB Lizenzfreie Online-Ressourcen |
| LEADER | 03925caa a2200517 4500 | ||
|---|---|---|---|
| 001 | 0-1580381340 | ||
| 003 | DE-627 | ||
| 005 | 20210815204112.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 180824s2018 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1038/s41598-018-30035-2 |2 doi | |
| 035 | |a (DE-627)1580381340 | ||
| 035 | |a (DE-576)510381340 | ||
| 035 | |a (DE-599)BSZ510381340 | ||
| 035 | |a (OCoLC)1264005784 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 100 | 1 | |a Lommel, Mark |e VerfasserIn |0 (DE-588)141467681 |0 (DE-627)627800149 |0 (DE-576)323794246 |4 aut | |
| 245 | 1 | 0 | |a Hydra mesoglea proteome identifies thrombospondin as a conserved component active in head organizer restriction |c Mark Lommel, Jennifer Strompen, Andrew L. Hellewell, Gnana Prakash Balasubramanian, Elena D. Christofidou, Andrew R. Thomson, Aimee L. Boyle, Derek N. Woolfson, Kane Puglisi, Markus Hartl, Thomas W. Holstein, Josephine C. Adams & Suat Özbek |
| 264 | 1 | |c 06 August 2018 | |
| 300 | |a 14 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 24.08.2018 | ||
| 520 | |a Thrombospondins (TSPs) are multidomain glycoproteins with complex matricellular functions in tissue homeostasis and remodeling. We describe a novel role of TSP as a Wnt signaling target in the basal eumetazoan Hydra. Proteome analysis identified Hydra magnipapillata TSP (HmTSP) as a major component of the cnidarian mesoglea. In general, the domain organization of cnidarian TSPs is related to the pentameric TSPs of bilaterians, and in phylogenetic analyses cnidarian TSPs formed a separate clade of high sequence diversity. HmTSP expression in polyps was restricted to the hypostomal tip and tentacle bases that harbor Wnt-regulated organizer tissues. In the hypostome, HmTSP- and Wnt3-expressing cells were identical or in close vicinity to each other, and regions of ectopic tentacle formation induced by pharmacological β-Catenin activation (Alsterpaullone) corresponded to foci of HmTSP expression. Chromatin immunoprecipitation (ChIP) confirmed binding of Hydra TCF to conserved elements in the HmTSP promotor region. Accordingly, β-Catenin knockdown by siRNAs reduced normal HmTSP expression at the head organizer. In contrast, knockdown of HmTSP expression led to increased numbers of ectopic organizers in Alsterpaullone-treated animals, indicating a negative regulatory function. Our data suggest an unexpected role for HmTSP as a feedback inhibitor of Wnt signaling during Hydra body axis patterning and maintenance. | ||
| 700 | 1 | |a Balasubramanian, Gnana Prakash |d 1981- |e VerfasserIn |0 (DE-588)14362671X |0 (DE-627)647708922 |0 (DE-576)338051554 |4 aut | |
| 700 | 1 | |a Holstein, Thomas W. |d 1953- |e VerfasserIn |0 (DE-588)1057984620 |0 (DE-627)796251053 |0 (DE-576)413989178 |4 aut | |
| 700 | 1 | |a Özbek, Suat |d 1968- |e VerfasserIn |0 (DE-588)122090470 |0 (DE-627)70576852X |0 (DE-576)293087024 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Scientific reports |d [London] : Macmillan Publishers Limited, part of Springer Nature, 2011 |g 8(2018) Artikel-Nummer 11753, 18 Seiten |h Online-Ressource |w (DE-627)663366712 |w (DE-600)2615211-3 |w (DE-576)346641179 |x 2045-2322 |7 nnns |
| 773 | 1 | 8 | |g volume:8 |g year:2018 |g extent:14 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1038/s41598-018-30035-2 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u https://www.nature.com/articles/s41598-018-30035-2 |x Verlag |z kostenfrei |3 Volltext |
| 936 | u | w | |d 8 |j 2018 |g 14 |y 8(2018) Artikel-Nummer 11753, 18 Seiten |
| 951 | |a AR | ||
| 856 | 4 | 0 | |u http://dx.doi.org/10.1038/s41598-018-30035-2 |9 LFER |
| 852 | |a LFER |z 2018-09-13T00:00:00Z | ||
| 970 | |c OD | ||
| 971 | |c EBOOK | ||
| 972 | |c EBOOK | ||
| 973 | |c Aufsatz | ||
| 935 | |a lfer | ||
| 900 | |a Prakash Balasubramanian, Gnana | ||
| 900 | |a Balasubramanian, Prakash G. | ||
| 900 | |a Holstein, Thomas | ||
| 980 | |a 1580381340 |b 0 |k 1580381340 |o 510381340 |c lfer | ||
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Hydra+mesoglea+proteome+identifies+thrombospondin+as+a+conserved+component+active+in+head+organizer+restriction&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.creator=Lommel%2C+Mark&rft.pub=&rft.format=Journal&rft.language=English&rft.issn=2045-2322 |
|---|
| _version_ | 1757949878220095488 |
|---|---|
| access_facet | Electronic Resources |
| author | Lommel, Mark, Balasubramanian, Gnana Prakash, Holstein, Thomas W., Özbek, Suat |
| author_facet | Lommel, Mark, Balasubramanian, Gnana Prakash, Holstein, Thomas W., Özbek, Suat |
| author_role | aut, aut, aut, aut |
| author_sort | Lommel, Mark |
| author_variant | m l ml, g p b gp gpb, t w h tw twh, s ö sö |
| callnumber-sort | |
| collection | lfer |
| container_reference | 8(2018) Artikel-Nummer 11753, 18 Seiten |
| container_title | Scientific reports |
| contents | Thrombospondins (TSPs) are multidomain glycoproteins with complex matricellular functions in tissue homeostasis and remodeling. We describe a novel role of TSP as a Wnt signaling target in the basal eumetazoan Hydra. Proteome analysis identified Hydra magnipapillata TSP (HmTSP) as a major component of the cnidarian mesoglea. In general, the domain organization of cnidarian TSPs is related to the pentameric TSPs of bilaterians, and in phylogenetic analyses cnidarian TSPs formed a separate clade of high sequence diversity. HmTSP expression in polyps was restricted to the hypostomal tip and tentacle bases that harbor Wnt-regulated organizer tissues. In the hypostome, HmTSP- and Wnt3-expressing cells were identical or in close vicinity to each other, and regions of ectopic tentacle formation induced by pharmacological β-Catenin activation (Alsterpaullone) corresponded to foci of HmTSP expression. Chromatin immunoprecipitation (ChIP) confirmed binding of Hydra TCF to conserved elements in the HmTSP promotor region. Accordingly, β-Catenin knockdown by siRNAs reduced normal HmTSP expression at the head organizer. In contrast, knockdown of HmTSP expression led to increased numbers of ectopic organizers in Alsterpaullone-treated animals, indicating a negative regulatory function. Our data suggest an unexpected role for HmTSP as a feedback inhibitor of Wnt signaling during Hydra body axis patterning and maintenance. |
| ctrlnum | (DE-627)1580381340, (DE-576)510381340, (DE-599)BSZ510381340, (OCoLC)1264005784 |
| doi_str_mv | 10.1038/s41598-018-30035-2 |
| facet_avail | Online, Free |
| finc_class_facet | not assigned |
| finc_id_str | 0021239580 |
| footnote | Gesehen am 24.08.2018 |
| format | ElectronicBookComponentPart |
| format_access_txtF_mv | Article, E-Article |
| format_de105 | Ebook |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_del152 | Buch |
| format_detail_txtF_mv | text-online-monograph-child |
| format_dezi4 | e-Book |
| format_finc | Article, E-Article |
| format_legacy | ElectronicBookPart |
| format_strict_txtF_mv | E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| hierarchy_parent_id | 0-663366712 |
| hierarchy_parent_title | Scientific reports |
| hierarchy_sequence | 8(2018) Artikel-Nummer 11753, 18 Seiten |
| hierarchy_top_id | 0-663366712 |
| hierarchy_top_title | Scientific reports |
| id | 0-1580381340 |
| illustrated | Not Illustrated |
| imprint | 06 August 2018 |
| imprint_str_mv | 06 August 2018 |
| institution | DE-D117, DE-105, LFER, DE-Ch1, DE-15, DE-14, DE-Zwi2 |
| is_hierarchy_id | 0-1580381340 |
| is_hierarchy_title | Hydra mesoglea proteome identifies thrombospondin as a conserved component active in head organizer restriction |
| isil_str_mv | LFER |
| issn | 2045-2322 |
| kxp_id_str | 1580381340 |
| language | English |
| last_indexed | 2023-02-16T01:40:47.537Z |
| marc024a_ct_mv | 10.1038/s41598-018-30035-2 |
| match_str | lommel2018hydramesogleaproteomeidentifiesthrombospondinasaconservedcomponentactiveinheadorganizerrestriction |
| mega_collection | Verbunddaten SWB, Lizenzfreie Online-Ressourcen |
| misc_de105 | EBOOK |
| multipart_link | 346641179 |
| multipart_part | (346641179)8(2018) Artikel-Nummer 11753, 18 Seiten |
| names_id_str_mv | (DE-588)141467681, (DE-627)627800149, (DE-576)323794246, (DE-588)14362671X, (DE-627)647708922, (DE-576)338051554, (DE-588)1057984620, (DE-627)796251053, (DE-576)413989178, (DE-588)122090470, (DE-627)70576852X, (DE-576)293087024 |
| oclc_num | 1264005784 |
| physical | 14 |
| publishDate | 06 August 2018 |
| publishDateSort | 2018 |
| publishPlace | |
| publisher | |
| record_format | marcfinc |
| record_id | 510381340 |
| recordtype | marcfinc |
| rvk_facet | No subject assigned |
| source_id | 0 |
| spelling | Lommel, Mark VerfasserIn (DE-588)141467681 (DE-627)627800149 (DE-576)323794246 aut, Hydra mesoglea proteome identifies thrombospondin as a conserved component active in head organizer restriction Mark Lommel, Jennifer Strompen, Andrew L. Hellewell, Gnana Prakash Balasubramanian, Elena D. Christofidou, Andrew R. Thomson, Aimee L. Boyle, Derek N. Woolfson, Kane Puglisi, Markus Hartl, Thomas W. Holstein, Josephine C. Adams & Suat Özbek, 06 August 2018, 14, Text txt rdacontent, Computermedien c rdamedia, Online-Ressource cr rdacarrier, Gesehen am 24.08.2018, Thrombospondins (TSPs) are multidomain glycoproteins with complex matricellular functions in tissue homeostasis and remodeling. We describe a novel role of TSP as a Wnt signaling target in the basal eumetazoan Hydra. Proteome analysis identified Hydra magnipapillata TSP (HmTSP) as a major component of the cnidarian mesoglea. In general, the domain organization of cnidarian TSPs is related to the pentameric TSPs of bilaterians, and in phylogenetic analyses cnidarian TSPs formed a separate clade of high sequence diversity. HmTSP expression in polyps was restricted to the hypostomal tip and tentacle bases that harbor Wnt-regulated organizer tissues. In the hypostome, HmTSP- and Wnt3-expressing cells were identical or in close vicinity to each other, and regions of ectopic tentacle formation induced by pharmacological β-Catenin activation (Alsterpaullone) corresponded to foci of HmTSP expression. Chromatin immunoprecipitation (ChIP) confirmed binding of Hydra TCF to conserved elements in the HmTSP promotor region. Accordingly, β-Catenin knockdown by siRNAs reduced normal HmTSP expression at the head organizer. In contrast, knockdown of HmTSP expression led to increased numbers of ectopic organizers in Alsterpaullone-treated animals, indicating a negative regulatory function. Our data suggest an unexpected role for HmTSP as a feedback inhibitor of Wnt signaling during Hydra body axis patterning and maintenance., Balasubramanian, Gnana Prakash 1981- VerfasserIn (DE-588)14362671X (DE-627)647708922 (DE-576)338051554 aut, Holstein, Thomas W. 1953- VerfasserIn (DE-588)1057984620 (DE-627)796251053 (DE-576)413989178 aut, Özbek, Suat 1968- VerfasserIn (DE-588)122090470 (DE-627)70576852X (DE-576)293087024 aut, Enthalten in Scientific reports [London] : Macmillan Publishers Limited, part of Springer Nature, 2011 8(2018) Artikel-Nummer 11753, 18 Seiten Online-Ressource (DE-627)663366712 (DE-600)2615211-3 (DE-576)346641179 2045-2322 nnns, volume:8 year:2018 extent:14, http://dx.doi.org/10.1038/s41598-018-30035-2 Verlag Resolving-System kostenfrei Volltext, https://www.nature.com/articles/s41598-018-30035-2 Verlag kostenfrei Volltext, http://dx.doi.org/10.1038/s41598-018-30035-2 LFER, LFER 2018-09-13T00:00:00Z |
| spellingShingle | Lommel, Mark, Balasubramanian, Gnana Prakash, Holstein, Thomas W., Özbek, Suat, Hydra mesoglea proteome identifies thrombospondin as a conserved component active in head organizer restriction, Thrombospondins (TSPs) are multidomain glycoproteins with complex matricellular functions in tissue homeostasis and remodeling. We describe a novel role of TSP as a Wnt signaling target in the basal eumetazoan Hydra. Proteome analysis identified Hydra magnipapillata TSP (HmTSP) as a major component of the cnidarian mesoglea. In general, the domain organization of cnidarian TSPs is related to the pentameric TSPs of bilaterians, and in phylogenetic analyses cnidarian TSPs formed a separate clade of high sequence diversity. HmTSP expression in polyps was restricted to the hypostomal tip and tentacle bases that harbor Wnt-regulated organizer tissues. In the hypostome, HmTSP- and Wnt3-expressing cells were identical or in close vicinity to each other, and regions of ectopic tentacle formation induced by pharmacological β-Catenin activation (Alsterpaullone) corresponded to foci of HmTSP expression. Chromatin immunoprecipitation (ChIP) confirmed binding of Hydra TCF to conserved elements in the HmTSP promotor region. Accordingly, β-Catenin knockdown by siRNAs reduced normal HmTSP expression at the head organizer. In contrast, knockdown of HmTSP expression led to increased numbers of ectopic organizers in Alsterpaullone-treated animals, indicating a negative regulatory function. Our data suggest an unexpected role for HmTSP as a feedback inhibitor of Wnt signaling during Hydra body axis patterning and maintenance. |
| swb_id_str | 510381340 |
| title | Hydra mesoglea proteome identifies thrombospondin as a conserved component active in head organizer restriction |
| title_auth | Hydra mesoglea proteome identifies thrombospondin as a conserved component active in head organizer restriction |
| title_full | Hydra mesoglea proteome identifies thrombospondin as a conserved component active in head organizer restriction Mark Lommel, Jennifer Strompen, Andrew L. Hellewell, Gnana Prakash Balasubramanian, Elena D. Christofidou, Andrew R. Thomson, Aimee L. Boyle, Derek N. Woolfson, Kane Puglisi, Markus Hartl, Thomas W. Holstein, Josephine C. Adams & Suat Özbek |
| title_fullStr | Hydra mesoglea proteome identifies thrombospondin as a conserved component active in head organizer restriction Mark Lommel, Jennifer Strompen, Andrew L. Hellewell, Gnana Prakash Balasubramanian, Elena D. Christofidou, Andrew R. Thomson, Aimee L. Boyle, Derek N. Woolfson, Kane Puglisi, Markus Hartl, Thomas W. Holstein, Josephine C. Adams & Suat Özbek |
| title_full_unstemmed | Hydra mesoglea proteome identifies thrombospondin as a conserved component active in head organizer restriction Mark Lommel, Jennifer Strompen, Andrew L. Hellewell, Gnana Prakash Balasubramanian, Elena D. Christofidou, Andrew R. Thomson, Aimee L. Boyle, Derek N. Woolfson, Kane Puglisi, Markus Hartl, Thomas W. Holstein, Josephine C. Adams & Suat Özbek |
| title_in_hierarchy | Hydra mesoglea proteome identifies thrombospondin as a conserved component active in head organizer restriction / Mark Lommel, Jennifer Strompen, Andrew L. Hellewell, Gnana Prakash Balasubramanian, Elena D. Christofidou, Andrew R. Thomson, Aimee L. Boyle, Derek N. Woolfson, Kane Puglisi, Markus Hartl, Thomas W. Holstein, Josephine C. Adams & Suat Özbek, |
| title_short | Hydra mesoglea proteome identifies thrombospondin as a conserved component active in head organizer restriction |
| title_sort | hydra mesoglea proteome identifies thrombospondin as a conserved component active in head organizer restriction |
| url | http://dx.doi.org/10.1038/s41598-018-30035-2, https://www.nature.com/articles/s41598-018-30035-2 |