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Tumor-expressed inducible nitric oxide synthase controls induction of functional myeloid-derived suppressor cells through modulation of vascular endothelial growth factor release
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| Veröffentlicht in: | The journal of immunology 188(2012), 11, Seite 5365-5376 |
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| Personen und Körperschaften: | , , |
| Titel: | Tumor-expressed inducible nitric oxide synthase controls induction of functional myeloid-derived suppressor cells through modulation of vascular endothelial growth factor release/ Padmini Jayaraman, Falguni Parikh, Esther Lopez-Rivera, Yared Hailemichael, Amelia Clark, Ge Ma, David Cannan, Marcel Ramacher, Masashi Kato, Willem W. Overwijk, Shu-Hsia Chen, Viktor Y. Umansky, Andrew G. Sikora |
| Format: | E-Book-Kapitel |
| Sprache: | Englisch |
| veröffentlicht: |
23 April 2012
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| Gesamtaufnahme: |
: The journal of immunology, 188(2012), 11, Seite 5365-5376
, volume:188 |
| Quelle: | Verbunddaten SWB Lizenzfreie Online-Ressourcen |
| Zusammenfassung: | Inducible NO synthase (iNOS) is a hallmark of chronic inflammation that is also overexpressed in melanoma and other cancers. Whereas iNOS is a known effector of myeloid-derived suppressor cell (MDSC)-mediated immunosuppression, its pivotal position at the interface of inflammation and cancer also makes it an attractive candidate regulator of MDSC recruitment. We hypothesized that tumor-expressed iNOS controls MDSC accumulation and acquisition of suppressive activity in melanoma. CD11b+GR1+ MDSC derived from mouse bone marrow cells cultured in the presence of MT-RET-1 mouse melanoma cells or conditioned supernatants expressed STAT3 and reactive oxygen species (ROS) and efficiently suppressed T cell proliferation. Inhibition of tumor-expressed iNOS with the small molecule inhibitor L-NIL blocked accumulation of STAT3/ROS-expressing MDSC, and abolished their suppressive function. Experiments with vascular endothelial growth factor (VEGF)-depleting Ab and recombinant VEGF identified a key role for VEGF in the iNOS-dependent induction of MDSC. These findings were further validated in mice bearing transplantable MT-RET-1 melanoma, in which L-NIL normalized elevated serum VEGF levels; downregulated activated STAT3 and ROS production in MDSC; and reversed tumor-mediated immunosuppression. These beneficial effects were not observed in iNOS knockout mice, suggesting L-NIL acts primarily on tumor- rather than host-expressed iNOS to regulate MDSC function. A significant decrease in tumor growth and a trend toward increased tumor-infiltrating CD8+ T cells were also observed in MT-RET transgenic mice bearing spontaneous tumors. These data suggest a critical role for tumor-expressed iNOS in the recruitment and induction of functional MDSC by modulation of tumor VEGF secretion and upregulation of STAT3 and ROS in MDSC. |
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| Beschreibung: |
Prepublished online 23 April 2012 Gesehen am 20.08.2018 |
| Umfang: | 12 |
| ISSN: |
1550-6606
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| DOI: | 10.4049/jimmunol.1103553 |