Eintrag weiter verarbeiten
Verfügbar über Online-Ressource

A genome-wide RNA interference screen identifies caspase 4 as a factor required for tumor necrosis factor alpha signaling

Gespeichert in:

Veröffentlicht in: Molecular and cellular biology 32(2012), 17, Seite 3372-3381
Personen und Körperschaften: Nickles, Dorothee (VerfasserIn), Oliver Metzig, Marie (VerfasserIn), Boutros, Michael (VerfasserIn)
Titel: A genome-wide RNA interference screen identifies caspase 4 as a factor required for tumor necrosis factor alpha signaling/ Dorothee Nickles, Christina Falschlehner, Marie Metzig, and Michael Boutros
Format: E-Book-Kapitel
Sprache: Englisch
veröffentlicht:
2012
Gesamtaufnahme: : Molecular and cellular biology, 32(2012), 17, Seite 3372-3381
, volume:32
Quelle: Verbunddaten SWB
Lizenzfreie Online-Ressourcen
LEADER 03548caa a2200529 4500
001 0-1576408213
003 DE-627
005 20220814164318.0
007 cr uuu---uuuuu
008 180615s2012 xx |||||o 00| ||eng c
024 7 |a 10.1128/MCB.06739-11  |2 doi 
035 |a (DE-627)1576408213 
035 |a (DE-576)506408213 
035 |a (DE-599)BSZ506408213 
035 |a (OCoLC)1341011789 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
100 1 |a Nickles, Dorothee  |e VerfasserIn  |0 (DE-588)140704043  |0 (DE-627)620896299  |0 (DE-576)319766888  |4 aut 
245 1 2 |a A genome-wide RNA interference screen identifies caspase 4 as a factor required for tumor necrosis factor alpha signaling  |c Dorothee Nickles, Christina Falschlehner, Marie Metzig, and Michael Boutros 
264 1 |c 2012 
300 |a 10 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Published ahead of print 25 June 2012 
500 |a Gesehen am 15.06.2018 
520 |a Tumor necrosis factor alpha (TNF-α) is a potent inflammatory cytokine secreted upon cellular stress as well as immunological stimuli and is implicated in the pathology of inflammatory diseases and cancer. The therapeutic potential of modifying TNF-α pathway activity has been realized in several diseases, and antagonists of TNF-α have reached clinical applications. While much progress in the understanding of signaling downstream of the TNF-α receptor complex has been made, the compendium of factors required for signal transduction is still not complete. In order to find novel regulators of proinflammatory signaling induced by TNF-α, we conducted a genome-wide small interfering RNA screen in human cells. We identified several new candidate modulators of TNF-α signaling, which were confirmed in independent experiments. Specifically, we show that caspase 4 is required for the induction of NF-κB activity, while it appears to be dispensable for the activation of the Jun N-terminal protein kinase signaling branch. Taken together, our experiments identify caspase 4 as a novel regulator of TNF-α-induced NF-κB signaling that is required for the activation of IκB kinase. We further provide the genome-wide RNA interference data set as a compendium in a format compliant with minimum information about an interfering RNA experiment (MAIRE). 
700 1 |a Oliver Metzig, Marie  |d 1985-  |e VerfasserIn  |0 (DE-588)1023029898  |0 (DE-627)717349624  |0 (DE-576)366289691  |4 aut 
700 1 |a Boutros, Michael  |e VerfasserIn  |0 (DE-588)1023031132  |0 (DE-627)717350045  |0 (DE-576)366291831  |4 aut 
773 0 8 |i Enthalten in  |t Molecular and cellular biology  |d Washington, DC : Soc., 1981  |g 32(2012), 17, Seite 3372-3381  |h Online-Ressource  |w (DE-627)269533656  |w (DE-600)1474919-1  |w (DE-576)088704181  |x 1098-5549  |7 nnns 
773 1 8 |g volume:32  |g year:2012  |g number:17  |g pages:3372-3381  |g extent:10 
856 4 0 |u http://dx.doi.org/10.1128/MCB.06739-11  |x Verlag  |x Resolving-System  |z kostenfrei  |3 Volltext 
856 4 0 |u http://mcb.asm.org/content/32/17/3372  |x Verlag  |z kostenfrei  |3 Volltext 
936 u w |d 32  |j 2012  |e 17  |h 3372-3381  |g 10 
951 |a AR 
856 4 0 |u http://dx.doi.org/10.1128/MCB.06739-11  |9 LFER 
852 |a LFER  |z 2018-07-10T00:00:00Z 
970 |c OD 
971 |c EBOOK 
972 |c EBOOK 
973 |c Aufsatz 
935 |a lfer 
900 |a Metzig, Marie 
900 |a Oliver-Metzig, Marie 
900 |a Metzig, Marie Oliver 
951 |b XA-DE 
980 |a 1576408213  |b 0  |k 1576408213  |o 506408213  |c lfer 
openURL url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=A+genome-wide+RNA+interference+screen+identifies+caspase+4+as+a+factor+required+for+tumor+necrosis+factor+alpha+signaling&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.creator=Nickles%2C+Dorothee&rft.pub=&rft.format=Journal&rft.language=English&rft.issn=1098-5549
SOLR
_version_ 1757949667505602560
access_facet Electronic Resources
author Nickles, Dorothee, Oliver Metzig, Marie, Boutros, Michael
author_facet Nickles, Dorothee, Oliver Metzig, Marie, Boutros, Michael
author_role aut, aut, aut
author_sort Nickles, Dorothee
author_variant d n dn, m m o mm mmo, m b mb
callnumber-sort
collection lfer
container_reference 32(2012), 17, Seite 3372-3381
container_title Molecular and cellular biology
contents Tumor necrosis factor alpha (TNF-α) is a potent inflammatory cytokine secreted upon cellular stress as well as immunological stimuli and is implicated in the pathology of inflammatory diseases and cancer. The therapeutic potential of modifying TNF-α pathway activity has been realized in several diseases, and antagonists of TNF-α have reached clinical applications. While much progress in the understanding of signaling downstream of the TNF-α receptor complex has been made, the compendium of factors required for signal transduction is still not complete. In order to find novel regulators of proinflammatory signaling induced by TNF-α, we conducted a genome-wide small interfering RNA screen in human cells. We identified several new candidate modulators of TNF-α signaling, which were confirmed in independent experiments. Specifically, we show that caspase 4 is required for the induction of NF-κB activity, while it appears to be dispensable for the activation of the Jun N-terminal protein kinase signaling branch. Taken together, our experiments identify caspase 4 as a novel regulator of TNF-α-induced NF-κB signaling that is required for the activation of IκB kinase. We further provide the genome-wide RNA interference data set as a compendium in a format compliant with minimum information about an interfering RNA experiment (MAIRE).
ctrlnum (DE-627)1576408213, (DE-576)506408213, (DE-599)BSZ506408213, (OCoLC)1341011789
doi_str_mv 10.1128/MCB.06739-11
facet_avail Online, Free
finc_class_facet not assigned
finc_id_str 0021171179
footnote Published ahead of print 25 June 2012, Gesehen am 15.06.2018
format ElectronicBookComponentPart
format_access_txtF_mv Article, E-Article
format_de105 Ebook
format_de14 Article, E-Article
format_de15 Article, E-Article
format_del152 Buch
format_detail_txtF_mv text-online-monograph-child
format_dezi4 e-Book
format_finc Article, E-Article
format_legacy ElectronicBookPart
format_strict_txtF_mv E-Article
geogr_code not assigned
geogr_code_person Germany
hierarchy_parent_id 0-269533656
hierarchy_parent_title Molecular and cellular biology
hierarchy_sequence 32(2012), 17, Seite 3372-3381
hierarchy_top_id 0-269533656
hierarchy_top_title Molecular and cellular biology
id 0-1576408213
illustrated Not Illustrated
imprint 2012
imprint_str_mv 2012
institution DE-D117, DE-105, LFER, DE-Ch1, DE-15, DE-14, DE-Zwi2
is_hierarchy_id 0-1576408213
is_hierarchy_title A genome-wide RNA interference screen identifies caspase 4 as a factor required for tumor necrosis factor alpha signaling
isil_str_mv LFER
issn 1098-5549
kxp_id_str 1576408213
language English
last_indexed 2023-02-16T01:37:27.229Z
marc024a_ct_mv 10.1128/MCB.06739-11
match_str nickles2012agenomewidernainterferencescreenidentifiescaspase4asafactorrequiredfortumornecrosisfactoralphasignaling
mega_collection Verbunddaten SWB, Lizenzfreie Online-Ressourcen
misc_de105 EBOOK
multipart_link 088704181
multipart_part (088704181)32(2012), 17, Seite 3372-3381
names_id_str_mv (DE-588)140704043, (DE-627)620896299, (DE-576)319766888, (DE-588)1023029898, (DE-627)717349624, (DE-576)366289691, (DE-588)1023031132, (DE-627)717350045, (DE-576)366291831
oclc_num 1341011789
physical 10
publishDate 2012
publishDateSort 2012
publishPlace
publisher
record_format marcfinc
record_id 506408213
recordtype marcfinc
rvk_facet No subject assigned
source_id 0
spelling Nickles, Dorothee VerfasserIn (DE-588)140704043 (DE-627)620896299 (DE-576)319766888 aut, A genome-wide RNA interference screen identifies caspase 4 as a factor required for tumor necrosis factor alpha signaling Dorothee Nickles, Christina Falschlehner, Marie Metzig, and Michael Boutros, 2012, 10, Text txt rdacontent, Computermedien c rdamedia, Online-Ressource cr rdacarrier, Published ahead of print 25 June 2012, Gesehen am 15.06.2018, Tumor necrosis factor alpha (TNF-α) is a potent inflammatory cytokine secreted upon cellular stress as well as immunological stimuli and is implicated in the pathology of inflammatory diseases and cancer. The therapeutic potential of modifying TNF-α pathway activity has been realized in several diseases, and antagonists of TNF-α have reached clinical applications. While much progress in the understanding of signaling downstream of the TNF-α receptor complex has been made, the compendium of factors required for signal transduction is still not complete. In order to find novel regulators of proinflammatory signaling induced by TNF-α, we conducted a genome-wide small interfering RNA screen in human cells. We identified several new candidate modulators of TNF-α signaling, which were confirmed in independent experiments. Specifically, we show that caspase 4 is required for the induction of NF-κB activity, while it appears to be dispensable for the activation of the Jun N-terminal protein kinase signaling branch. Taken together, our experiments identify caspase 4 as a novel regulator of TNF-α-induced NF-κB signaling that is required for the activation of IκB kinase. We further provide the genome-wide RNA interference data set as a compendium in a format compliant with minimum information about an interfering RNA experiment (MAIRE)., Oliver Metzig, Marie 1985- VerfasserIn (DE-588)1023029898 (DE-627)717349624 (DE-576)366289691 aut, Boutros, Michael VerfasserIn (DE-588)1023031132 (DE-627)717350045 (DE-576)366291831 aut, Enthalten in Molecular and cellular biology Washington, DC : Soc., 1981 32(2012), 17, Seite 3372-3381 Online-Ressource (DE-627)269533656 (DE-600)1474919-1 (DE-576)088704181 1098-5549 nnns, volume:32 year:2012 number:17 pages:3372-3381 extent:10, http://dx.doi.org/10.1128/MCB.06739-11 Verlag Resolving-System kostenfrei Volltext, http://mcb.asm.org/content/32/17/3372 Verlag kostenfrei Volltext, http://dx.doi.org/10.1128/MCB.06739-11 LFER, LFER 2018-07-10T00:00:00Z
spellingShingle Nickles, Dorothee, Oliver Metzig, Marie, Boutros, Michael, A genome-wide RNA interference screen identifies caspase 4 as a factor required for tumor necrosis factor alpha signaling, Tumor necrosis factor alpha (TNF-α) is a potent inflammatory cytokine secreted upon cellular stress as well as immunological stimuli and is implicated in the pathology of inflammatory diseases and cancer. The therapeutic potential of modifying TNF-α pathway activity has been realized in several diseases, and antagonists of TNF-α have reached clinical applications. While much progress in the understanding of signaling downstream of the TNF-α receptor complex has been made, the compendium of factors required for signal transduction is still not complete. In order to find novel regulators of proinflammatory signaling induced by TNF-α, we conducted a genome-wide small interfering RNA screen in human cells. We identified several new candidate modulators of TNF-α signaling, which were confirmed in independent experiments. Specifically, we show that caspase 4 is required for the induction of NF-κB activity, while it appears to be dispensable for the activation of the Jun N-terminal protein kinase signaling branch. Taken together, our experiments identify caspase 4 as a novel regulator of TNF-α-induced NF-κB signaling that is required for the activation of IκB kinase. We further provide the genome-wide RNA interference data set as a compendium in a format compliant with minimum information about an interfering RNA experiment (MAIRE).
swb_id_str 506408213
title A genome-wide RNA interference screen identifies caspase 4 as a factor required for tumor necrosis factor alpha signaling
title_auth A genome-wide RNA interference screen identifies caspase 4 as a factor required for tumor necrosis factor alpha signaling
title_full A genome-wide RNA interference screen identifies caspase 4 as a factor required for tumor necrosis factor alpha signaling Dorothee Nickles, Christina Falschlehner, Marie Metzig, and Michael Boutros
title_fullStr A genome-wide RNA interference screen identifies caspase 4 as a factor required for tumor necrosis factor alpha signaling Dorothee Nickles, Christina Falschlehner, Marie Metzig, and Michael Boutros
title_full_unstemmed A genome-wide RNA interference screen identifies caspase 4 as a factor required for tumor necrosis factor alpha signaling Dorothee Nickles, Christina Falschlehner, Marie Metzig, and Michael Boutros
title_in_hierarchy A genome-wide RNA interference screen identifies caspase 4 as a factor required for tumor necrosis factor alpha signaling / Dorothee Nickles, Christina Falschlehner, Marie Metzig, and Michael Boutros,
title_short A genome-wide RNA interference screen identifies caspase 4 as a factor required for tumor necrosis factor alpha signaling
title_sort genome wide rna interference screen identifies caspase 4 as a factor required for tumor necrosis factor alpha signaling
url http://dx.doi.org/10.1128/MCB.06739-11, http://mcb.asm.org/content/32/17/3372