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Functional fingerprinting of human mesenchymal stem cells using high-throughput RNAi screening
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| Veröffentlicht in: | Genome medicine 7(2015) Artikel-Nummer 46, 13 Seiten |
|---|---|
| Personen und Körperschaften: | , , , , |
| Titel: | Functional fingerprinting of human mesenchymal stem cells using high-throughput RNAi screening/ Gerrit Erdmann, Michael Suchanek, Patrick Horn, Fabian Graf, Christian Volz, Thomas Horn, Xian Zhang, Wolfgang Wagner, Anthony D. Ho and Michael Boutros |
| Format: | E-Book-Kapitel |
| Sprache: | Englisch |
| veröffentlicht: |
17 May 2015
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| Gesamtaufnahme: |
: Genome medicine, 7(2015) Artikel-Nummer 46, 13 Seiten
, volume:7 |
| Schlagwörter: | |
| Quelle: | Verbunddaten SWB Lizenzfreie Online-Ressourcen |
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| 520 | |a Mesenchymal stem cells (MSCs) are promising candidates for cellular therapies ranging from tissue repair in regenerative medicine to immunomodulation in graft versus host disease after allogeneic transplantation or in autoimmune diseases. Nonetheless, progress has been hampered by their enormous phenotypic as well as functional heterogeneity and the lack of uniform standards and guidelines for quality control. In this study, we describe a method to perform cellular phenotyping by high-throughput RNA interference in primary human bone marrow MSCs. We have shown that despite heterogeneity of MSC populations, robust functional assays can be established that are suitable for high-throughput and high-content screening. We profiled primary human MSCs against human fibroblasts. Network analysis showed a kinome fingerprint that differs from human primary fibroblasts as well as fibroblast cell lines. In conclusion, this study shows that high-throughput screening in primary human MSCs can be reliably used for kinome fingerprinting. | ||
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| author | Erdmann, Gerrit, Horn, Patrick, Wagner, Wolfgang, Ho, Anthony Dick, Boutros, Michael |
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| contents | Mesenchymal stem cells (MSCs) are promising candidates for cellular therapies ranging from tissue repair in regenerative medicine to immunomodulation in graft versus host disease after allogeneic transplantation or in autoimmune diseases. Nonetheless, progress has been hampered by their enormous phenotypic as well as functional heterogeneity and the lack of uniform standards and guidelines for quality control. In this study, we describe a method to perform cellular phenotyping by high-throughput RNA interference in primary human bone marrow MSCs. We have shown that despite heterogeneity of MSC populations, robust functional assays can be established that are suitable for high-throughput and high-content screening. We profiled primary human MSCs against human fibroblasts. Network analysis showed a kinome fingerprint that differs from human primary fibroblasts as well as fibroblast cell lines. In conclusion, this study shows that high-throughput screening in primary human MSCs can be reliably used for kinome fingerprinting. |
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| spelling | Erdmann, Gerrit VerfasserIn (DE-588)1044338261 (DE-627)771803613 (DE-576)396891020 aut, Functional fingerprinting of human mesenchymal stem cells using high-throughput RNAi screening Gerrit Erdmann, Michael Suchanek, Patrick Horn, Fabian Graf, Christian Volz, Thomas Horn, Xian Zhang, Wolfgang Wagner, Anthony D. Ho and Michael Boutros, 17 May 2015, 13, Text txt rdacontent, Computermedien c rdamedia, Online-Ressource cr rdacarrier, Gesehen am 01.02.2018, Mesenchymal stem cells (MSCs) are promising candidates for cellular therapies ranging from tissue repair in regenerative medicine to immunomodulation in graft versus host disease after allogeneic transplantation or in autoimmune diseases. Nonetheless, progress has been hampered by their enormous phenotypic as well as functional heterogeneity and the lack of uniform standards and guidelines for quality control. In this study, we describe a method to perform cellular phenotyping by high-throughput RNA interference in primary human bone marrow MSCs. We have shown that despite heterogeneity of MSC populations, robust functional assays can be established that are suitable for high-throughput and high-content screening. We profiled primary human MSCs against human fibroblasts. Network analysis showed a kinome fingerprint that differs from human primary fibroblasts as well as fibroblast cell lines. In conclusion, this study shows that high-throughput screening in primary human MSCs can be reliably used for kinome fingerprinting., Cell Cycle Profile, Fibroblast Cell Line, Human Bone Marrow, Laser Scanning Cytometry, Primary Fibroblast, Horn, Patrick VerfasserIn (DE-588)143037390 (DE-627)704371251 (DE-576)334708311 aut, Wagner, Wolfgang 1973- VerfasserIn (DE-588)124106803 (DE-627)706539451 (DE-576)294022635 aut, Ho, Anthony Dick 1948- VerfasserIn (DE-588)108692477 (DE-627)504904205 (DE-576)19010466X aut, Boutros, Michael VerfasserIn (DE-588)1023031132 (DE-627)717350045 (DE-576)366291831 aut, Enthalten in Genome medicine London : BioMed Central, 2009 7(2015) Artikel-Nummer 46, 13 Seiten Online-Ressource (DE-627)594424275 (DE-600)2484394-5 (DE-576)304547956 1756-994X nnns, volume:7 year:2015 extent:13, http://dx.doi.org/10.1186/s13073-015-0170-2 Verlag Resolving-System kostenfrei Volltext, https://doi.org/10.1186/s13073-015-0170-2 Verlag kostenfrei Volltext, http://dx.doi.org/10.1186/s13073-015-0170-2 LFER, LFER 2018-03-07T00:00:00Z |
| spellingShingle | Erdmann, Gerrit, Horn, Patrick, Wagner, Wolfgang, Ho, Anthony Dick, Boutros, Michael, Functional fingerprinting of human mesenchymal stem cells using high-throughput RNAi screening, Mesenchymal stem cells (MSCs) are promising candidates for cellular therapies ranging from tissue repair in regenerative medicine to immunomodulation in graft versus host disease after allogeneic transplantation or in autoimmune diseases. Nonetheless, progress has been hampered by their enormous phenotypic as well as functional heterogeneity and the lack of uniform standards and guidelines for quality control. In this study, we describe a method to perform cellular phenotyping by high-throughput RNA interference in primary human bone marrow MSCs. We have shown that despite heterogeneity of MSC populations, robust functional assays can be established that are suitable for high-throughput and high-content screening. We profiled primary human MSCs against human fibroblasts. Network analysis showed a kinome fingerprint that differs from human primary fibroblasts as well as fibroblast cell lines. In conclusion, this study shows that high-throughput screening in primary human MSCs can be reliably used for kinome fingerprinting., Cell Cycle Profile, Fibroblast Cell Line, Human Bone Marrow, Laser Scanning Cytometry, Primary Fibroblast |
| swb_id_str | 498015416 |
| title | Functional fingerprinting of human mesenchymal stem cells using high-throughput RNAi screening |
| title_auth | Functional fingerprinting of human mesenchymal stem cells using high-throughput RNAi screening |
| title_full | Functional fingerprinting of human mesenchymal stem cells using high-throughput RNAi screening Gerrit Erdmann, Michael Suchanek, Patrick Horn, Fabian Graf, Christian Volz, Thomas Horn, Xian Zhang, Wolfgang Wagner, Anthony D. Ho and Michael Boutros |
| title_fullStr | Functional fingerprinting of human mesenchymal stem cells using high-throughput RNAi screening Gerrit Erdmann, Michael Suchanek, Patrick Horn, Fabian Graf, Christian Volz, Thomas Horn, Xian Zhang, Wolfgang Wagner, Anthony D. Ho and Michael Boutros |
| title_full_unstemmed | Functional fingerprinting of human mesenchymal stem cells using high-throughput RNAi screening Gerrit Erdmann, Michael Suchanek, Patrick Horn, Fabian Graf, Christian Volz, Thomas Horn, Xian Zhang, Wolfgang Wagner, Anthony D. Ho and Michael Boutros |
| title_in_hierarchy | Functional fingerprinting of human mesenchymal stem cells using high-throughput RNAi screening / Gerrit Erdmann, Michael Suchanek, Patrick Horn, Fabian Graf, Christian Volz, Thomas Horn, Xian Zhang, Wolfgang Wagner, Anthony D. Ho and Michael Boutros, |
| title_short | Functional fingerprinting of human mesenchymal stem cells using high-throughput RNAi screening |
| title_sort | functional fingerprinting of human mesenchymal stem cells using high throughput rnai screening |
| topic | Cell Cycle Profile, Fibroblast Cell Line, Human Bone Marrow, Laser Scanning Cytometry, Primary Fibroblast |
| topic_facet | Cell Cycle Profile, Fibroblast Cell Line, Human Bone Marrow, Laser Scanning Cytometry, Primary Fibroblast |
| url | http://dx.doi.org/10.1186/s13073-015-0170-2, https://doi.org/10.1186/s13073-015-0170-2 |