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GLC and GLC-MS analysis of thiophene derivatives in plants and in in vitro cultures of Tagetes patula L. (Asteraceae)
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| Veröffentlicht in: | Zeitschrift für Naturforschung / A 57(2014), 1-2, Seite 63-71 |
|---|---|
| Personen und Körperschaften: | , , |
| Titel: | GLC and GLC-MS analysis of thiophene derivatives in plants and in in vitro cultures of Tagetes patula L. (Asteraceae)/ Lilla Margl, Andreas Tei, István Gyurján and Michael Wink |
| Format: | E-Book-Kapitel |
| Sprache: | Englisch |
| veröffentlicht: |
02.06.2014
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| Gesamtaufnahme: |
: Zeitschrift für Naturforschung / A, 57(2014), 1-2, Seite 63-71
, volume:57 |
| Schlagwörter: | |
| Quelle: | Verbunddaten SWB Lizenzfreie Online-Ressourcen |
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| 245 | 1 | 0 | |a GLC and GLC-MS analysis of thiophene derivatives in plants and in in vitro cultures of Tagetes patula L. (Asteraceae) |c Lilla Margl, Andreas Tei, István Gyurján and Michael Wink |
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| 520 | |a The occurrence of thiophenic compounds in diverse plant organs and in in vitro root-, callus- and cell suspension cultures of Tagetes patula cv. Carmen was investigated using capillary GLC and GLC-MS. The separation of thiophenes by capillary GLC and the group specific MS fragmentation with the typical sulfur isotope peaks allowed the unequivocal assignment of individual thiophenes in complex mixtures, even when occurring in traces and in the presence of different geometrical isomers. The extracts of Tagetes patula cv. Carmen contained the following 8 thiophene compounds: 5-(3-buten-1-ynyl)-2,2′-bithienyl (BBT), 5′-methyl-5-(3-buten-1-ynyl)-2,2′-bithienyl (MeBBT), 5-(1-pentynyl)-2,2′-bithienyl (PBT), 5-(4-hydroxy-1-butynyl)-2,2′-bithienyl (BBTOH), 2,2′,5,2″-terthienyl (α-T), 5-(4-acetoxy- 1-butynyl)-2,2′-bithienyl (BBTOAc), 5-methylaceto-5′-(3-buten-1-ynyl)-2,2′-bithienyl (AcOCH2BBT), and 5-(3,4-diacetoxy-1-butynyl)-2,2′-bithienyl (BBT(OAc)2). The most complex thiophene profile, including the less common PBT was detected in aerial parts of freshly harvested plant material. Under in vitro conditions only the root cultures, but not callus or cell suspension cultures produced substantial amounts of irregular thiophenes confirming that roots are the main site of thiophene biosynthesis. | ||
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| 650 | 4 | |a Tagetes patula | |
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| 700 | 1 | |a Wink, Michael |d 1951- |e VerfasserIn |0 (DE-588)124618146 |0 (DE-627)363448519 |0 (DE-576)164659390 |4 aut | |
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| contents | The occurrence of thiophenic compounds in diverse plant organs and in in vitro root-, callus- and cell suspension cultures of Tagetes patula cv. Carmen was investigated using capillary GLC and GLC-MS. The separation of thiophenes by capillary GLC and the group specific MS fragmentation with the typical sulfur isotope peaks allowed the unequivocal assignment of individual thiophenes in complex mixtures, even when occurring in traces and in the presence of different geometrical isomers. The extracts of Tagetes patula cv. Carmen contained the following 8 thiophene compounds: 5-(3-buten-1-ynyl)-2,2′-bithienyl (BBT), 5′-methyl-5-(3-buten-1-ynyl)-2,2′-bithienyl (MeBBT), 5-(1-pentynyl)-2,2′-bithienyl (PBT), 5-(4-hydroxy-1-butynyl)-2,2′-bithienyl (BBTOH), 2,2′,5,2″-terthienyl (α-T), 5-(4-acetoxy- 1-butynyl)-2,2′-bithienyl (BBTOAc), 5-methylaceto-5′-(3-buten-1-ynyl)-2,2′-bithienyl (AcOCH2BBT), and 5-(3,4-diacetoxy-1-butynyl)-2,2′-bithienyl (BBT(OAc)2). The most complex thiophene profile, including the less common PBT was detected in aerial parts of freshly harvested plant material. Under in vitro conditions only the root cultures, but not callus or cell suspension cultures produced substantial amounts of irregular thiophenes confirming that roots are the main site of thiophene biosynthesis. |
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| spelling | Römisch-Margl, Lilla VerfasserIn (DE-588)114080572X (DE-627)898700108 (DE-576)313075344 aut, GLC and GLC-MS analysis of thiophene derivatives in plants and in in vitro cultures of Tagetes patula L. (Asteraceae) Lilla Margl, Andreas Tei, István Gyurján and Michael Wink, 02.06.2014, 9, Text txt rdacontent, Computermedien c rdamedia, Online-Ressource cr rdacarrier, Gesehen am 04.10.2017, The occurrence of thiophenic compounds in diverse plant organs and in in vitro root-, callus- and cell suspension cultures of Tagetes patula cv. Carmen was investigated using capillary GLC and GLC-MS. The separation of thiophenes by capillary GLC and the group specific MS fragmentation with the typical sulfur isotope peaks allowed the unequivocal assignment of individual thiophenes in complex mixtures, even when occurring in traces and in the presence of different geometrical isomers. The extracts of Tagetes patula cv. Carmen contained the following 8 thiophene compounds: 5-(3-buten-1-ynyl)-2,2′-bithienyl (BBT), 5′-methyl-5-(3-buten-1-ynyl)-2,2′-bithienyl (MeBBT), 5-(1-pentynyl)-2,2′-bithienyl (PBT), 5-(4-hydroxy-1-butynyl)-2,2′-bithienyl (BBTOH), 2,2′,5,2″-terthienyl (α-T), 5-(4-acetoxy- 1-butynyl)-2,2′-bithienyl (BBTOAc), 5-methylaceto-5′-(3-buten-1-ynyl)-2,2′-bithienyl (AcOCH2BBT), and 5-(3,4-diacetoxy-1-butynyl)-2,2′-bithienyl (BBT(OAc)2). The most complex thiophene profile, including the less common PBT was detected in aerial parts of freshly harvested plant material. Under in vitro conditions only the root cultures, but not callus or cell suspension cultures produced substantial amounts of irregular thiophenes confirming that roots are the main site of thiophene biosynthesis., Root Biosynthesis, Tagetes patula, Thiophenes, Tei, Andreas VerfasserIn (DE-588)1119822602 (DE-627)872899608 (DE-576)182823504 aut, Wink, Michael 1951- VerfasserIn (DE-588)124618146 (DE-627)363448519 (DE-576)164659390 aut, Enthalten in Zeitschrift für Naturforschung / A Berlin : De Gruyter, 1947 57(2014), 1-2, Seite 63-71 Online-Ressource (DE-627)348586299 (DE-600)2079610-9 (DE-576)101177984 1865-7109 nnns, volume:57 year:2014 number:1-2 pages:63-71 extent:9, http://dx.doi.org/10.1515/znc-2002-1-211 Verlag Resolving-System kostenfrei Volltext, http://dx.doi.org/10.1515/znc-2002-1-211 LFER, LFER 2017-10-10T00:00:00Z |
| spellingShingle | Römisch-Margl, Lilla, Tei, Andreas, Wink, Michael, GLC and GLC-MS analysis of thiophene derivatives in plants and in in vitro cultures of Tagetes patula L. (Asteraceae), The occurrence of thiophenic compounds in diverse plant organs and in in vitro root-, callus- and cell suspension cultures of Tagetes patula cv. Carmen was investigated using capillary GLC and GLC-MS. The separation of thiophenes by capillary GLC and the group specific MS fragmentation with the typical sulfur isotope peaks allowed the unequivocal assignment of individual thiophenes in complex mixtures, even when occurring in traces and in the presence of different geometrical isomers. The extracts of Tagetes patula cv. Carmen contained the following 8 thiophene compounds: 5-(3-buten-1-ynyl)-2,2′-bithienyl (BBT), 5′-methyl-5-(3-buten-1-ynyl)-2,2′-bithienyl (MeBBT), 5-(1-pentynyl)-2,2′-bithienyl (PBT), 5-(4-hydroxy-1-butynyl)-2,2′-bithienyl (BBTOH), 2,2′,5,2″-terthienyl (α-T), 5-(4-acetoxy- 1-butynyl)-2,2′-bithienyl (BBTOAc), 5-methylaceto-5′-(3-buten-1-ynyl)-2,2′-bithienyl (AcOCH2BBT), and 5-(3,4-diacetoxy-1-butynyl)-2,2′-bithienyl (BBT(OAc)2). The most complex thiophene profile, including the less common PBT was detected in aerial parts of freshly harvested plant material. Under in vitro conditions only the root cultures, but not callus or cell suspension cultures produced substantial amounts of irregular thiophenes confirming that roots are the main site of thiophene biosynthesis., Root Biosynthesis, Tagetes patula, Thiophenes |
| swb_id_str | 494056479 |
| title | GLC and GLC-MS analysis of thiophene derivatives in plants and in in vitro cultures of Tagetes patula L. (Asteraceae) |
| title_auth | GLC and GLC-MS analysis of thiophene derivatives in plants and in in vitro cultures of Tagetes patula L. (Asteraceae) |
| title_full | GLC and GLC-MS analysis of thiophene derivatives in plants and in in vitro cultures of Tagetes patula L. (Asteraceae) Lilla Margl, Andreas Tei, István Gyurján and Michael Wink |
| title_fullStr | GLC and GLC-MS analysis of thiophene derivatives in plants and in in vitro cultures of Tagetes patula L. (Asteraceae) Lilla Margl, Andreas Tei, István Gyurján and Michael Wink |
| title_full_unstemmed | GLC and GLC-MS analysis of thiophene derivatives in plants and in in vitro cultures of Tagetes patula L. (Asteraceae) Lilla Margl, Andreas Tei, István Gyurján and Michael Wink |
| title_in_hierarchy | GLC and GLC-MS analysis of thiophene derivatives in plants and in in vitro cultures of Tagetes patula L. (Asteraceae) / Lilla Margl, Andreas Tei, István Gyurján and Michael Wink, |
| title_short | GLC and GLC-MS analysis of thiophene derivatives in plants and in in vitro cultures of Tagetes patula L. (Asteraceae) |
| title_sort | glc and glc ms analysis of thiophene derivatives in plants and in in vitro cultures of tagetes patula l asteraceae |
| topic | Root Biosynthesis, Tagetes patula, Thiophenes |
| topic_facet | Root Biosynthesis, Tagetes patula, Thiophenes |
| url | http://dx.doi.org/10.1515/znc-2002-1-211 |