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Definedcd conditions for the isolation and expansion of basal prostate progenitor cells of mouse and human origin
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| Veröffentlicht in: | Stem cell reports year:2015; volume:4; pages:503-518; extent:16; 4(2015), 3, Seite 503-518; number:3 |
|---|---|
| Personen und Körperschaften: | , , , , |
| Titel: | Definedcd conditions for the isolation and expansion of basal prostate progenitor cells of mouse and human origin/ Thomas Höfner, Christian Eisen, Corinna Klein, Teresa Rigo-Watermeier, Stephan M. Goeppinger, Anna Jauch, Brigitte Schoell, Vanessa Vogel, Elisa Noll, Wilko Weichert, Irène Baccelli, Anja Schillert, Steve Wagner, Sascha Pahernik, Martin R. Sprick, and Andreas Trumpp |
| Format: | E-Book-Kapitel |
| Sprache: | Englisch |
| veröffentlicht: |
February 19, 2015
|
| Gesamtaufnahme: |
: Stem cell reports, 4(2015), 3, Seite 503-518
, volume:4 |
| Quelle: | Verbunddaten SWB Lizenzfreie Online-Ressourcen |
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| 520 | |a Methods to isolate and culture primary prostate epithelial stem/progenitor cells (PESCs) have proven difficult and ineffective. Here, we present a method to grow and expand both murine and human basal PESCs long term in serum- and feeder-free conditions. The method enriches for adherent mouse basal PESCs with a Lin−SCA-1+CD49f+TROP2high phenotype. Progesterone and sodium selenite are additionally required for the growth of human Lin−CD49f+TROP2high PESCs. The gene-expression profiles of expanded basal PESCs show similarities to ESCs, and NF-kB function is critical for epithelial differentiation of sphere-cultured PESCs. When transplanted in combination with urogenital sinus mesenchyme, expanded mouse and human PESCs generate ectopic prostatic tubules, demonstrating their stem cell activity in vivo. This novel method will facilitate the molecular, genomic, and functional characterization of normal and pathologic prostate glands of mouse and human origin. | ||
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| author | Höfner, Thomas, Macher-Göppinger, Stephan, Jauch, Anna, Weichert, Wilko, Pahernik, Sascha |
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| contents | Methods to isolate and culture primary prostate epithelial stem/progenitor cells (PESCs) have proven difficult and ineffective. Here, we present a method to grow and expand both murine and human basal PESCs long term in serum- and feeder-free conditions. The method enriches for adherent mouse basal PESCs with a Lin−SCA-1+CD49f+TROP2high phenotype. Progesterone and sodium selenite are additionally required for the growth of human Lin−CD49f+TROP2high PESCs. The gene-expression profiles of expanded basal PESCs show similarities to ESCs, and NF-kB function is critical for epithelial differentiation of sphere-cultured PESCs. When transplanted in combination with urogenital sinus mesenchyme, expanded mouse and human PESCs generate ectopic prostatic tubules, demonstrating their stem cell activity in vivo. This novel method will facilitate the molecular, genomic, and functional characterization of normal and pathologic prostate glands of mouse and human origin. |
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| spelling | Höfner, Thomas 1979- VerfasserIn (DE-588)132286963 (DE-627)520406907 (DE-576)299053156 aut, Definedcd conditions for the isolation and expansion of basal prostate progenitor cells of mouse and human origin Thomas Höfner, Christian Eisen, Corinna Klein, Teresa Rigo-Watermeier, Stephan M. Goeppinger, Anna Jauch, Brigitte Schoell, Vanessa Vogel, Elisa Noll, Wilko Weichert, Irène Baccelli, Anja Schillert, Steve Wagner, Sascha Pahernik, Martin R. Sprick, and Andreas Trumpp, February 19, 2015, 16, Text txt rdacontent, Computermedien c rdamedia, Online-Ressource cr rdacarrier, Gesehen am 30.08.2017, Methods to isolate and culture primary prostate epithelial stem/progenitor cells (PESCs) have proven difficult and ineffective. Here, we present a method to grow and expand both murine and human basal PESCs long term in serum- and feeder-free conditions. The method enriches for adherent mouse basal PESCs with a Lin−SCA-1+CD49f+TROP2high phenotype. Progesterone and sodium selenite are additionally required for the growth of human Lin−CD49f+TROP2high PESCs. The gene-expression profiles of expanded basal PESCs show similarities to ESCs, and NF-kB function is critical for epithelial differentiation of sphere-cultured PESCs. When transplanted in combination with urogenital sinus mesenchyme, expanded mouse and human PESCs generate ectopic prostatic tubules, demonstrating their stem cell activity in vivo. This novel method will facilitate the molecular, genomic, and functional characterization of normal and pathologic prostate glands of mouse and human origin., Macher-Göppinger, Stephan VerfasserIn (DE-588)1022394061 (DE-627)716963299 (DE-576)365275034 aut, Jauch, Anna VerfasserIn (DE-588)1025525140 (DE-627)722525362 (DE-576)168357496 aut, Weichert, Wilko 1970-2023 VerfasserIn (DE-588)123509106 (DE-627)56134650X (DE-576)293740461 aut, Pahernik, Sascha 1968- VerfasserIn (DE-588)122063163 (DE-627)081713495 (DE-576)293073147 aut, Enthalten in Stem cell reports Maryland Heights, MO : Cell Press, 2013 4(2015), 3, Seite 503-518 Online-Ressource (DE-627)749730862 (DE-600)2720528-9 (DE-576)383773474 2213-6711 nnns, volume:4 year:2015 number:3 pages:503-518 extent:16, http://dx.doi.org/10.1016/j.stemcr.2015.01.015 Verlag Resolving-System kostenfrei Volltext, http://www.cell.com/stem-cell-reports/abstract/S2213-6711(15)00036-3 Verlag kostenfrei Volltext, http://dx.doi.org/10.1016/j.stemcr.2015.01.015 LFER, LFER epn:2980229377 2017-09-14T00:00:00Z |
| spellingShingle | Höfner, Thomas, Macher-Göppinger, Stephan, Jauch, Anna, Weichert, Wilko, Pahernik, Sascha, Definedcd conditions for the isolation and expansion of basal prostate progenitor cells of mouse and human origin, Methods to isolate and culture primary prostate epithelial stem/progenitor cells (PESCs) have proven difficult and ineffective. Here, we present a method to grow and expand both murine and human basal PESCs long term in serum- and feeder-free conditions. The method enriches for adherent mouse basal PESCs with a Lin−SCA-1+CD49f+TROP2high phenotype. Progesterone and sodium selenite are additionally required for the growth of human Lin−CD49f+TROP2high PESCs. The gene-expression profiles of expanded basal PESCs show similarities to ESCs, and NF-kB function is critical for epithelial differentiation of sphere-cultured PESCs. When transplanted in combination with urogenital sinus mesenchyme, expanded mouse and human PESCs generate ectopic prostatic tubules, demonstrating their stem cell activity in vivo. This novel method will facilitate the molecular, genomic, and functional characterization of normal and pathologic prostate glands of mouse and human origin. |
| swb_id_str | 492896755 |
| title | Definedcd conditions for the isolation and expansion of basal prostate progenitor cells of mouse and human origin |
| title_auth | Definedcd conditions for the isolation and expansion of basal prostate progenitor cells of mouse and human origin |
| title_full | Definedcd conditions for the isolation and expansion of basal prostate progenitor cells of mouse and human origin Thomas Höfner, Christian Eisen, Corinna Klein, Teresa Rigo-Watermeier, Stephan M. Goeppinger, Anna Jauch, Brigitte Schoell, Vanessa Vogel, Elisa Noll, Wilko Weichert, Irène Baccelli, Anja Schillert, Steve Wagner, Sascha Pahernik, Martin R. Sprick, and Andreas Trumpp |
| title_fullStr | Definedcd conditions for the isolation and expansion of basal prostate progenitor cells of mouse and human origin Thomas Höfner, Christian Eisen, Corinna Klein, Teresa Rigo-Watermeier, Stephan M. Goeppinger, Anna Jauch, Brigitte Schoell, Vanessa Vogel, Elisa Noll, Wilko Weichert, Irène Baccelli, Anja Schillert, Steve Wagner, Sascha Pahernik, Martin R. Sprick, and Andreas Trumpp |
| title_full_unstemmed | Definedcd conditions for the isolation and expansion of basal prostate progenitor cells of mouse and human origin Thomas Höfner, Christian Eisen, Corinna Klein, Teresa Rigo-Watermeier, Stephan M. Goeppinger, Anna Jauch, Brigitte Schoell, Vanessa Vogel, Elisa Noll, Wilko Weichert, Irène Baccelli, Anja Schillert, Steve Wagner, Sascha Pahernik, Martin R. Sprick, and Andreas Trumpp |
| title_in_hierarchy | Definedcd conditions for the isolation and expansion of basal prostate progenitor cells of mouse and human origin / Thomas Höfner, Christian Eisen, Corinna Klein, Teresa Rigo-Watermeier, Stephan M. Goeppinger, Anna Jauch, Brigitte Schoell, Vanessa Vogel, Elisa Noll, Wilko Weichert, Irène Baccelli, Anja Schillert, Steve Wagner, Sascha Pahernik, Martin R. Sprick, and Andreas Trumpp, |
| title_short | Definedcd conditions for the isolation and expansion of basal prostate progenitor cells of mouse and human origin |
| title_sort | definedcd conditions for the isolation and expansion of basal prostate progenitor cells of mouse and human origin |
| title_unstemmed | Definedcd conditions for the isolation and expansion of basal prostate progenitor cells of mouse and human origin |
| url | http://dx.doi.org/10.1016/j.stemcr.2015.01.015, http://www.cell.com/stem-cell-reports/abstract/S2213-6711(15)00036-3 |
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