Eintrag weiter verarbeiten
CD74 and CD44 Expression on CTCs in Cancer Patients with Brain Metastasis
Gespeichert in:
| Zeitschriftentitel: | International Journal of Molecular Sciences |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , , , , , , , , , , , , , , , |
| In: | International Journal of Molecular Sciences, 22, 2021, 13, S. 6993 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
MDPI AG
|
| Schlagwörter: |
| author_facet |
Loreth, Desiree Schuette, Moritz Zinke, Jenny Mohme, Malte Piffko, Andras Schneegans, Svenja Stadler, Julia Janning, Melanie Loges, Sonja Joosse, Simon A. Lamszus, Katrin Westphal, Manfred Müller, Volkmar Glatzel, Markus Matschke, Jakob Gebhardt, Christoffer Schneider, Stefan W. Belczacka, Iwona Volkmer, Beate Greinert, Rüdiger Yaspo, Marie-Laure Harter, Patrick N. Pantel, Klaus Wikman, Harriet Loreth, Desiree Schuette, Moritz Zinke, Jenny Mohme, Malte Piffko, Andras Schneegans, Svenja Stadler, Julia Janning, Melanie Loges, Sonja Joosse, Simon A. Lamszus, Katrin Westphal, Manfred Müller, Volkmar Glatzel, Markus Matschke, Jakob Gebhardt, Christoffer Schneider, Stefan W. Belczacka, Iwona Volkmer, Beate Greinert, Rüdiger Yaspo, Marie-Laure Harter, Patrick N. Pantel, Klaus Wikman, Harriet |
|---|---|
| author |
Loreth, Desiree Schuette, Moritz Zinke, Jenny Mohme, Malte Piffko, Andras Schneegans, Svenja Stadler, Julia Janning, Melanie Loges, Sonja Joosse, Simon A. Lamszus, Katrin Westphal, Manfred Müller, Volkmar Glatzel, Markus Matschke, Jakob Gebhardt, Christoffer Schneider, Stefan W. Belczacka, Iwona Volkmer, Beate Greinert, Rüdiger Yaspo, Marie-Laure Harter, Patrick N. Pantel, Klaus Wikman, Harriet |
| spellingShingle |
Loreth, Desiree Schuette, Moritz Zinke, Jenny Mohme, Malte Piffko, Andras Schneegans, Svenja Stadler, Julia Janning, Melanie Loges, Sonja Joosse, Simon A. Lamszus, Katrin Westphal, Manfred Müller, Volkmar Glatzel, Markus Matschke, Jakob Gebhardt, Christoffer Schneider, Stefan W. Belczacka, Iwona Volkmer, Beate Greinert, Rüdiger Yaspo, Marie-Laure Harter, Patrick N. Pantel, Klaus Wikman, Harriet International Journal of Molecular Sciences CD74 and CD44 Expression on CTCs in Cancer Patients with Brain Metastasis Inorganic Chemistry Organic Chemistry Physical and Theoretical Chemistry Computer Science Applications Spectroscopy Molecular Biology General Medicine Catalysis |
| author_sort |
loreth, desiree |
| spelling |
Loreth, Desiree Schuette, Moritz Zinke, Jenny Mohme, Malte Piffko, Andras Schneegans, Svenja Stadler, Julia Janning, Melanie Loges, Sonja Joosse, Simon A. Lamszus, Katrin Westphal, Manfred Müller, Volkmar Glatzel, Markus Matschke, Jakob Gebhardt, Christoffer Schneider, Stefan W. Belczacka, Iwona Volkmer, Beate Greinert, Rüdiger Yaspo, Marie-Laure Harter, Patrick N. Pantel, Klaus Wikman, Harriet 1422-0067 MDPI AG Inorganic Chemistry Organic Chemistry Physical and Theoretical Chemistry Computer Science Applications Spectroscopy Molecular Biology General Medicine Catalysis http://dx.doi.org/10.3390/ijms22136993 <jats:p>Up to 40% of advance lung, melanoma and breast cancer patients suffer from brain metastases (BM) with increasing incidence. Here, we assessed whether circulating tumor cells (CTCs) in peripheral blood can serve as a disease surrogate, focusing on CD44 and CD74 expression as prognostic markers for BM. We show that a size-based microfluidic approach in combination with a semi-automated cell recognition system are well suited for CTC detection in BM patients and allow further characterization of tumor cells potentially derived from BM. CTCs were found in 50% (7/14) of breast cancer, 50% (9/18) of non-small cell lung cancer (NSCLC) and 36% (4/11) of melanoma patients. The next-generation sequencing (NGS) analysis of nine single CTCs from one breast cancer patient revealed three different CNV profile groups as well as a resistance causing ERS1 mutation. CD44 and CD74 were expressed on most CTCs and their expression was strongly correlated, whereas matched breast cancer BM tissues were much less frequently expressing CD44 and CD74 (negative in 46% and 54%, respectively). Thus, plasticity of CD44 and CD74 expression during trafficking of CTCs in the circulation might be the result of adaptation strategies.</jats:p> CD74 and CD44 Expression on CTCs in Cancer Patients with Brain Metastasis International Journal of Molecular Sciences |
| doi_str_mv |
10.3390/ijms22136993 |
| facet_avail |
Online Free |
| finc_class_facet |
Physik Informatik Biologie Chemie und Pharmazie |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMzM5MC9pam1zMjIxMzY5OTM |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMzM5MC9pam1zMjIxMzY5OTM |
| institution |
DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 |
| imprint |
MDPI AG, 2021 |
| imprint_str_mv |
MDPI AG, 2021 |
| issn |
1422-0067 |
| issn_str_mv |
1422-0067 |
| language |
English |
| mega_collection |
MDPI AG (CrossRef) |
| match_str |
loreth2021cd74andcd44expressiononctcsincancerpatientswithbrainmetastasis |
| publishDateSort |
2021 |
| publisher |
MDPI AG |
| recordtype |
ai |
| record_format |
ai |
| series |
International Journal of Molecular Sciences |
| source_id |
49 |
| title |
CD74 and CD44 Expression on CTCs in Cancer Patients with Brain Metastasis |
| title_unstemmed |
CD74 and CD44 Expression on CTCs in Cancer Patients with Brain Metastasis |
| title_full |
CD74 and CD44 Expression on CTCs in Cancer Patients with Brain Metastasis |
| title_fullStr |
CD74 and CD44 Expression on CTCs in Cancer Patients with Brain Metastasis |
| title_full_unstemmed |
CD74 and CD44 Expression on CTCs in Cancer Patients with Brain Metastasis |
| title_short |
CD74 and CD44 Expression on CTCs in Cancer Patients with Brain Metastasis |
| title_sort |
cd74 and cd44 expression on ctcs in cancer patients with brain metastasis |
| topic |
Inorganic Chemistry Organic Chemistry Physical and Theoretical Chemistry Computer Science Applications Spectroscopy Molecular Biology General Medicine Catalysis |
| url |
http://dx.doi.org/10.3390/ijms22136993 |
| publishDate |
2021 |
| physical |
6993 |
| description |
<jats:p>Up to 40% of advance lung, melanoma and breast cancer patients suffer from brain metastases (BM) with increasing incidence. Here, we assessed whether circulating tumor cells (CTCs) in peripheral blood can serve as a disease surrogate, focusing on CD44 and CD74 expression as prognostic markers for BM. We show that a size-based microfluidic approach in combination with a semi-automated cell recognition system are well suited for CTC detection in BM patients and allow further characterization of tumor cells potentially derived from BM. CTCs were found in 50% (7/14) of breast cancer, 50% (9/18) of non-small cell lung cancer (NSCLC) and 36% (4/11) of melanoma patients. The next-generation sequencing (NGS) analysis of nine single CTCs from one breast cancer patient revealed three different CNV profile groups as well as a resistance causing ERS1 mutation. CD44 and CD74 were expressed on most CTCs and their expression was strongly correlated, whereas matched breast cancer BM tissues were much less frequently expressing CD44 and CD74 (negative in 46% and 54%, respectively). Thus, plasticity of CD44 and CD74 expression during trafficking of CTCs in the circulation might be the result of adaptation strategies.</jats:p> |
| container_issue |
13 |
| container_start_page |
0 |
| container_title |
International Journal of Molecular Sciences |
| container_volume |
22 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792348408254562304 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T18:09:56.781Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=CD74+and+CD44+Expression+on+CTCs+in+Cancer+Patients+with+Brain+Metastasis&rft.date=2021-06-29&genre=article&issn=1422-0067&volume=22&issue=13&pages=6993&jtitle=International+Journal+of+Molecular+Sciences&atitle=CD74+and+CD44+Expression+on+CTCs+in+Cancer+Patients+with+Brain+Metastasis&aulast=Wikman&aufirst=Harriet&rft_id=info%3Adoi%2F10.3390%2Fijms22136993&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792348408254562304 |
| author | Loreth, Desiree, Schuette, Moritz, Zinke, Jenny, Mohme, Malte, Piffko, Andras, Schneegans, Svenja, Stadler, Julia, Janning, Melanie, Loges, Sonja, Joosse, Simon A., Lamszus, Katrin, Westphal, Manfred, Müller, Volkmar, Glatzel, Markus, Matschke, Jakob, Gebhardt, Christoffer, Schneider, Stefan W., Belczacka, Iwona, Volkmer, Beate, Greinert, Rüdiger, Yaspo, Marie-Laure, Harter, Patrick N., Pantel, Klaus, Wikman, Harriet |
| author_facet | Loreth, Desiree, Schuette, Moritz, Zinke, Jenny, Mohme, Malte, Piffko, Andras, Schneegans, Svenja, Stadler, Julia, Janning, Melanie, Loges, Sonja, Joosse, Simon A., Lamszus, Katrin, Westphal, Manfred, Müller, Volkmar, Glatzel, Markus, Matschke, Jakob, Gebhardt, Christoffer, Schneider, Stefan W., Belczacka, Iwona, Volkmer, Beate, Greinert, Rüdiger, Yaspo, Marie-Laure, Harter, Patrick N., Pantel, Klaus, Wikman, Harriet, Loreth, Desiree, Schuette, Moritz, Zinke, Jenny, Mohme, Malte, Piffko, Andras, Schneegans, Svenja, Stadler, Julia, Janning, Melanie, Loges, Sonja, Joosse, Simon A., Lamszus, Katrin, Westphal, Manfred, Müller, Volkmar, Glatzel, Markus, Matschke, Jakob, Gebhardt, Christoffer, Schneider, Stefan W., Belczacka, Iwona, Volkmer, Beate, Greinert, Rüdiger, Yaspo, Marie-Laure, Harter, Patrick N., Pantel, Klaus, Wikman, Harriet |
| author_sort | loreth, desiree |
| container_issue | 13 |
| container_start_page | 0 |
| container_title | International Journal of Molecular Sciences |
| container_volume | 22 |
| description | <jats:p>Up to 40% of advance lung, melanoma and breast cancer patients suffer from brain metastases (BM) with increasing incidence. Here, we assessed whether circulating tumor cells (CTCs) in peripheral blood can serve as a disease surrogate, focusing on CD44 and CD74 expression as prognostic markers for BM. We show that a size-based microfluidic approach in combination with a semi-automated cell recognition system are well suited for CTC detection in BM patients and allow further characterization of tumor cells potentially derived from BM. CTCs were found in 50% (7/14) of breast cancer, 50% (9/18) of non-small cell lung cancer (NSCLC) and 36% (4/11) of melanoma patients. The next-generation sequencing (NGS) analysis of nine single CTCs from one breast cancer patient revealed three different CNV profile groups as well as a resistance causing ERS1 mutation. CD44 and CD74 were expressed on most CTCs and their expression was strongly correlated, whereas matched breast cancer BM tissues were much less frequently expressing CD44 and CD74 (negative in 46% and 54%, respectively). Thus, plasticity of CD44 and CD74 expression during trafficking of CTCs in the circulation might be the result of adaptation strategies.</jats:p> |
| doi_str_mv | 10.3390/ijms22136993 |
| facet_avail | Online, Free |
| finc_class_facet | Physik, Informatik, Biologie, Chemie und Pharmazie |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMzM5MC9pam1zMjIxMzY5OTM |
| imprint | MDPI AG, 2021 |
| imprint_str_mv | MDPI AG, 2021 |
| institution | DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161 |
| issn | 1422-0067 |
| issn_str_mv | 1422-0067 |
| language | English |
| last_indexed | 2024-03-01T18:09:56.781Z |
| match_str | loreth2021cd74andcd44expressiononctcsincancerpatientswithbrainmetastasis |
| mega_collection | MDPI AG (CrossRef) |
| physical | 6993 |
| publishDate | 2021 |
| publishDateSort | 2021 |
| publisher | MDPI AG |
| record_format | ai |
| recordtype | ai |
| series | International Journal of Molecular Sciences |
| source_id | 49 |
| spelling | Loreth, Desiree Schuette, Moritz Zinke, Jenny Mohme, Malte Piffko, Andras Schneegans, Svenja Stadler, Julia Janning, Melanie Loges, Sonja Joosse, Simon A. Lamszus, Katrin Westphal, Manfred Müller, Volkmar Glatzel, Markus Matschke, Jakob Gebhardt, Christoffer Schneider, Stefan W. Belczacka, Iwona Volkmer, Beate Greinert, Rüdiger Yaspo, Marie-Laure Harter, Patrick N. Pantel, Klaus Wikman, Harriet 1422-0067 MDPI AG Inorganic Chemistry Organic Chemistry Physical and Theoretical Chemistry Computer Science Applications Spectroscopy Molecular Biology General Medicine Catalysis http://dx.doi.org/10.3390/ijms22136993 <jats:p>Up to 40% of advance lung, melanoma and breast cancer patients suffer from brain metastases (BM) with increasing incidence. Here, we assessed whether circulating tumor cells (CTCs) in peripheral blood can serve as a disease surrogate, focusing on CD44 and CD74 expression as prognostic markers for BM. We show that a size-based microfluidic approach in combination with a semi-automated cell recognition system are well suited for CTC detection in BM patients and allow further characterization of tumor cells potentially derived from BM. CTCs were found in 50% (7/14) of breast cancer, 50% (9/18) of non-small cell lung cancer (NSCLC) and 36% (4/11) of melanoma patients. The next-generation sequencing (NGS) analysis of nine single CTCs from one breast cancer patient revealed three different CNV profile groups as well as a resistance causing ERS1 mutation. CD44 and CD74 were expressed on most CTCs and their expression was strongly correlated, whereas matched breast cancer BM tissues were much less frequently expressing CD44 and CD74 (negative in 46% and 54%, respectively). Thus, plasticity of CD44 and CD74 expression during trafficking of CTCs in the circulation might be the result of adaptation strategies.</jats:p> CD74 and CD44 Expression on CTCs in Cancer Patients with Brain Metastasis International Journal of Molecular Sciences |
| spellingShingle | Loreth, Desiree, Schuette, Moritz, Zinke, Jenny, Mohme, Malte, Piffko, Andras, Schneegans, Svenja, Stadler, Julia, Janning, Melanie, Loges, Sonja, Joosse, Simon A., Lamszus, Katrin, Westphal, Manfred, Müller, Volkmar, Glatzel, Markus, Matschke, Jakob, Gebhardt, Christoffer, Schneider, Stefan W., Belczacka, Iwona, Volkmer, Beate, Greinert, Rüdiger, Yaspo, Marie-Laure, Harter, Patrick N., Pantel, Klaus, Wikman, Harriet, International Journal of Molecular Sciences, CD74 and CD44 Expression on CTCs in Cancer Patients with Brain Metastasis, Inorganic Chemistry, Organic Chemistry, Physical and Theoretical Chemistry, Computer Science Applications, Spectroscopy, Molecular Biology, General Medicine, Catalysis |
| title | CD74 and CD44 Expression on CTCs in Cancer Patients with Brain Metastasis |
| title_full | CD74 and CD44 Expression on CTCs in Cancer Patients with Brain Metastasis |
| title_fullStr | CD74 and CD44 Expression on CTCs in Cancer Patients with Brain Metastasis |
| title_full_unstemmed | CD74 and CD44 Expression on CTCs in Cancer Patients with Brain Metastasis |
| title_short | CD74 and CD44 Expression on CTCs in Cancer Patients with Brain Metastasis |
| title_sort | cd74 and cd44 expression on ctcs in cancer patients with brain metastasis |
| title_unstemmed | CD74 and CD44 Expression on CTCs in Cancer Patients with Brain Metastasis |
| topic | Inorganic Chemistry, Organic Chemistry, Physical and Theoretical Chemistry, Computer Science Applications, Spectroscopy, Molecular Biology, General Medicine, Catalysis |
| url | http://dx.doi.org/10.3390/ijms22136993 |