%0 Electronic Article
%A Ge, Yan and Rikihisa, Yasuko
%I American Society for Microbiology
%D 2007
%D 2007
%G English
%@ 0019-9567
%@ 1098-5522
%~ Katalog der Westsächsischen Hochschule Zwickau
%T Surface-Exposed Proteins ofEhrlichia chaffeensis
%V 75
%J Infection and Immunity
%V 75
%N 8
%P 3833-3841
%U http://dx.doi.org/10.1128/iai.00188-07
%X ABSTRACTThe surface proteins ofEhrlichia chaffeensisprovide an important interface for pathogen-host interactions. To investigate the surface proteins ofE. chaffeensis, membrane-impermeable, cleavable Sulfo-NHS-SS-Biotin was used to label intact bacteria. The biotinylated bacterial surface proteins were isolated by streptavidin-agarose affinity purification. The affinity-captured proteins were separated by electrophoresis, and five relatively abundant protein bands containing immunoreactive proteins were subjected to capillary-liquid chromatography-nanospray tandem mass spectrometry analysis. Nineteen out of 22 OMP-1/P28 family proteins, including P28 (which previously was shown to be surface exposed), were detected inE. chaffeensiscultured in human monocytic leukemia THP-1 cells. For the first time, with the exception of P28 and P28-1, 17 OMP-1/P28 family proteins were demonstrated to be expressed at the protein level. The surface exposure of OMP-1A and OMP-1N was verified by immunofluorescence microscopy. OMP-1B was undetectable either by surface biotinylation or by Western blotting of the whole bacterial lysate, suggesting that it is not expressed byE. chaffeensiscultured in THP-1 cells. AdditionalE. chaffeensissurface proteins detected were OMP85, hypothetical protein ECH_0525 (here named Esp73), immunodominant surface protein gp47, and 11 other proteins. The identification ofE. chaffeensissurface-exposed proteins provides novel insights into theE. chaffeensissurface and lays the foundation for rational studies on pathogen-host interactions and vaccine development.
%Z https://www.katalog.fh-zwickau.de/Record/ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEyOC9pYWkuMDAxODgtMDc
%U https://www.katalog.fh-zwickau.de/Record/ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEyOC9pYWkuMDAxODgtMDc