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Characterization of phenolic compounds from Eugenia supra-axillaris leaf extract using HPLC-PDA-MS/MS and its antioxidant, anti-inflammatory, antipyretic and pain killing activitie...

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Veröffentlicht in: Scientific reports 9(2019) Artikel-Nummer 11122, 12 Seiten
Personen und Körperschaften: Hegazi, Nesrine M. (VerfasserIn), Sobeh, Mansour (VerfasserIn), Rezq, Samar (VerfasserIn), El-Raey, Mohamed A. (VerfasserIn), Dmirieh, Malak (VerfasserIn), El-Shazly, Assem M. (VerfasserIn), Mahmoud, Mona F. (VerfasserIn), Wink, Michael (VerfasserIn)
Titel: Characterization of phenolic compounds from Eugenia supra-axillaris leaf extract using HPLC-PDA-MS/MS and its antioxidant, anti-inflammatory, antipyretic and pain killing activities in vivo/ Nesrine M. Hegazi, Mansour Sobeh, Samar Rezq, Mohamed A. El-Raey, Malak Dmirieh, Assem M. El-Shazly, Mona F. Mahmoud & Michael Wink
Format: E-Book-Kapitel
Sprache: Englisch
veröffentlicht:
31 July 2019
Gesamtaufnahme: : Scientific reports, 9(2019) Artikel-Nummer 11122, 12 Seiten
, volume:9
Quelle: Verbunddaten SWB
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Zusammenfassung: Reactive oxygen species (ROS) are involved in the pathophysiology of several health disorders, among others inflammation. Polyphenols may modulate ROS related disorders. In this work, thirty-two phenolic compounds were tentatively identified in a leaf extract from Eugenia supra-axillaris Spring. ex Mart. using HPLC-MS/MS, five of which were also individually isolated and identified. The extract displayed a substantial in vitro antioxidant potential and was capable of decreasing ROS production and hsp-16.2 expression under oxidative stress conditions in vivo in the Caenorhabditis elegans model. Also, the extract showed higher inhibitory selectivity towards COX-2 than COX-1 in vitro with higher selectivity towards COX-2 than that of diclofenac. The extract also exhibited anti-inflammatory properties: It attenuated the edema thickness in a dose dependent fashion in carrageenan-induced hind-paw odema in rats. In addition, the extract reduced the carrageenan-induced leukocyte migration into the peritoneal cavity at the highest dose. Furthermore, the extract showed antipyretic and analgesic activities in a mouse model. Eugenia supra-axillaris appears to be a promising candidate in treating inflammation, pain and related oxidative stress diseases.
Beschreibung: Gesehen am 22.10.2019
ISSN: 2045-2322
DOI: 10.1038/s41598-019-46946-7