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Junb controls lymphatic vascular development in zebrafish via miR-182
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| Veröffentlicht in: | Scientific reports 5(2015) Artikel-Nummer 15007, 16 Seiten |
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| Personen und Körperschaften: | , , , |
| Titel: | Junb controls lymphatic vascular development in zebrafish via miR-182/ Kristin Kiesow, Katrin Bennewitz, Laura Gutierrez Miranda, Sandra J. Stoll, Bettina Hartenstein, Peter Angel, Jens Kroll, Marina Schorpp-Kistner |
| Format: | E-Book-Kapitel |
| Sprache: | Englisch |
| veröffentlicht: |
13 October 2015
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| Gesamtaufnahme: |
: Scientific reports, 5(2015) Artikel-Nummer 15007, 16 Seiten
, volume:5 |
| Quelle: | Verbunddaten SWB Lizenzfreie Online-Ressourcen |
| Zusammenfassung: | JUNB, a subunit of the AP-1 transcription factor complex, mediates gene regulation in response to a plethora of extracellular stimuli. Previously, JUNB was shown to act as a critical positive regulator of blood vessel development and homeostasis as well as a negative regulator of proliferation, inflammation and tumour growth. Here, we demonstrate that the oncogenic miR-182 is a novel JUNB target. Loss-of-function studies by morpholino-mediated knockdown and the CRISPR/Cas9 technology identify a novel function for both JUNB and its target miR-182 in lymphatic vascular development in zebrafish. Furthermore, we show that miR-182 attenuates foxo1 expression indicating that strictly balanced Foxo1 levels are required for proper lymphatic vascular development in zebrafish. In conclusion, our findings uncover with the Junb/miR-182/Foxo1 regulatory axis a novel key player in governing lymphatic vascular morphogenesis in zebrafish. |
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| Beschreibung: | Gesehen am 15.03.2018 |
| Umfang: | 16 |
| ISSN: |
2045-2322
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| DOI: | 10.1038/srep15007 |