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Measures of kidney function by minimally invasive techniques correlate with histological glomerular damage in SCID mice with adriamycin-induced nephropathy
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| Veröffentlicht in: | Scientific reports 5(2015) Artikel-Nummer 13601, 13 Seiten |
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| Personen und Körperschaften: | , |
| Titel: | Measures of kidney function by minimally invasive techniques correlate with histological glomerular damage in SCID mice with adriamycin-induced nephropathy/ Lauren Scarfe, Aleksandra Rak-Raszewska, Stefania Geraci, Darsy Darssan, Jack Sharkey, Jiaguo Huang, Neal C. Burton, David Mason, Parisa Ranjzad, Simon Kenny, Norbert Gretz, Raphaël Lévy, B. Kevin Park, Marta García-Fiñana, Adrian S. Woolf, Patricia Murray and Bettina Wilm |
| Format: | E-Book-Kapitel |
| Sprache: | Englisch |
| veröffentlicht: |
02 September 2015
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| Gesamtaufnahme: |
: Scientific reports, 5(2015) Artikel-Nummer 13601, 13 Seiten
, volume:5 |
| Quelle: | Verbunddaten SWB Lizenzfreie Online-Ressourcen |
| Zusammenfassung: | Maximising the use of preclinical murine models of progressive kidney disease as test beds for therapies ideally requires kidney function to be measured repeatedly in a safe, minimally invasive manner. To date, most studies of murine nephropathy depend on unreliable markers of renal physiological function, exemplified by measuring blood levels of creatinine and urea, and on various end points necessitating sacrifice of experimental animals to assess histological damage, thus counteracting the principles of Replacement, Refinement and Reduction. Here, we applied two novel minimally invasive techniques to measure kidney function in SCID mice with adriamycin-induced nephropathy. We employed i) a transcutaneous device that measures the half-life of intravenously administered FITC-sinistrin, a molecule cleared by glomerular filtration; and ii) multispectral optoacoustic tomography, a photoacoustic imaging device that directly visualises the clearance of the near infrared dye, IRDye 800CW carboxylate. Measurements with either technique showed a significant impairment of renal function in experimental animals versus controls, with significant correlations with the proportion of scarred glomeruli five weeks after induction of injury. These technologies provide clinically relevant functional data and should be widely adopted for testing the efficacies of novel therapies. Moreover, their use will also lead to a reduction in experimental animal numbers. |
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| Beschreibung: | Gesehen am 08.03.2018 |
| Umfang: | 13 |
| ISSN: |
2045-2322
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| DOI: | 10.1038/srep13601 |